Lipase (gene LipEMP48-D) from Micrococcus luteus EMP48-D

Enzyme Description

Species
Micrococcus luteus EMP48-D β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 451 amino acids
MAPARRPSRPLALAAAVAVVAGFGGPHAARAQESAPAPDAPEVSSAVADLSQPATTAEDARWNAARDALAAASVDDPFYLPPAVVPATPGALIRSEPAAFYLDPVRLVQVPAHATRIMYASQDAQGRPVAVTGTVLTPTAPWTGGGERPVIGYAVGTQGAADRCAPSRTLSTGAQYEGVGITSLLSRGHAVVVTDYEGLGTPGTHTYMVREAQAHAVLDAVRAAAQVSGSGVTSTAPVALAGYSQGGGAAAAAAELASDYAPELDLKGAYVGAPPADLVQVADRIDGGLYAAFLLYAMAGQLAAYDVDPATHHNETGLTALAGAAETCVAGSLAYAHLDSATLTHTGQDFPELVRSDAELAGILAEQRIGAPGRRPDVPVMIAHSVTDDVIPYRTGRDLGRRWCAEGARVRFDPLLTPTHVGGHVAALPRMGAFLDATLADRWTADSCGWF
Selfela Restu Adina et al. (2021) β€” Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification
Journal of Genetic Engineering and Biotechnology  Β· doi:10.1186/s43141-021-00155-w β†—  Β· Stability - Incubation
7 measurements ▼
Database ID
UniProt: A0A5B8YWX7 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris KM71

Experimental Data (7 measurements)

7 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase Ethanol 50% 100.0 102.8 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase Methanol 50% 100.0 101.7 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase 1-Butanol 50% 100.0 114.7 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase Isopropanol 50% 100.0 115.8 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase Acetonitrile 50% 100.0 102.2 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase n-Hexane 50% 100.0 41.2 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 40Β°C), measured by pH-metry (free fatty acid titration with NaOH) in aqueous phase n-Heptane 50% 100.0 83.9 % Incubation: ?, Assay: 11.5 mM citrate–phosphate buffer, 2.3% polyvinyl alcohol Incubation: ?, Assay: 5 Incubation: 40Β°C, Assay: 40Β°C 20% (v/v) Olive oil free fatty acids , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*