Cellulase (gene cel1029) from a soil metagenomic library

Enzyme Description

Species

Na (soil metagenomic library)

Extremophile

No but "thermostable" enzyme

EC Number

3.2.1.4 ↗

Sequence

Length: 261 amino acids

PLTVNGNRILANGEVRSLAGPSFFWSNTGWGAERFYNQDAVSWVKDDWNATLVRASLGVDGEGGYLEDPAGNKSRVVALVEAAIANDLYVIIDWHSHHAEDHTALAVDFFEEMAQTYGHHDNVIYEIYNEPLQISWSGTIKPYAETVISAIRAIDPDNLIVVGTPSWSQDVDAASWDPIQGYANIAYALHFYAGTHTQYLRDKAQTALNNGIALFVTEWGTVNANGDGGVAYDETQRWMDFLEANHISHAHWALNDKEKGA

Xiaoshen Zhao et al. (2021) — Screening, cloning, enzymatic properties of a novel thermostable cellulase enzyme, and its potential application on water hyacinth utilization

International Microbiology · doi:10.1007/s10123-021-00170-4 ↗ · Activity - Classical

12 measurements ▼

Database ID

UniParc: UPI00132F0C4E ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	1%	100	166	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	15%	100	115	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	30%	100	90	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	1%	100	116	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	15%	100	104	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	30%	100	95	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	1%	100	146	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	15%	100	111	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	30%	100	94	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	111	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	94	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	77	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	1%	100	166	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	15%	100	115	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	30%	100	90	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	1%	100	116	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	15%	100	104	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	30%	100	95	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	1%	100	146	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	15%	100	111	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Isopropanol	30%	100	94	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	111	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	94	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	77	%	40 mM Britton-Robinson buffer	6	55°C	1% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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