Laccase (gene BaCotA) from Bacillus stratosphericus BCMC2

Enzyme Description

Species
Bacillus stratosphericus BCMC2 β†—
Extremophile
No but "thermostable" enzyme
EC Number
1.10.3.2 β†—

Sequence

Length: 510 amino acids
MNLEKFVDELPIPEVAEPVKKNPRQTYYEIAMEEVFLKVHRDLPPTKLWTYNGSLPGPTIKANRNEKVKVKWMNKLPLKHFLPVDHTIHAGHHDEPEVKTVVHLHGGVTPASSDGYPEAWFSRDFEATGPFFEREVYEYPNHQQACTLWYHDHAMALTRLNVYAGLAGFYLISDAFEKSLELPKDEYDIPLMIMDRTFQEDGALFYPSRPNNTPEDSDLPDPSIVPFFCGETILVNGKVWPYLEVEPRKYRFRILNASNTRTYELHLDNDATILQIGSDGGFLPRPVHHQSFSIAPAERFDVIIDFSAYENKTIVLKNSAGCGQDVNPETDANIMQFKVTRPLKGRAAKTLRPIFKPLPPLRPSRADNERTLTLTGTQDKYGRPILLLDNQFWNDPVTENPRLGSVEVWNIVNPTRGTHPIHLHLVQFRVIDRRPFDTDIYQSTGEIVYTGPNEAPPLHEQGYKDTIQAHAGEVIRIIARFVPYSGRYVWHCHILEHEDYDMMRPMDIIQ
Yuanxi Xiao et al. (2021) β€” An alkaline thermostable laccase from termite gut associated strain of Bacillus stratosphericus
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2021.02.205 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: A0A7T1FRB0 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JM109

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 10% 100 121 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 50% 100 103 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 10% 100 122 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 50% 100 91 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 10% 100 125 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 50% 100 73 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 83 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100 18 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethylformamide (DMF) 10% 100 96 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethylformamide (DMF) 50% 100 68 % Incubation: ?, Assay: 100 mM sodium acetate buffer Incubation: ?, Assay: 5 Incubation: 25Β°C, Assay: 60Β°C 0.5 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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