Protease (gene HlyA) from Halococcus salifodinae DSM 8989

Enzyme Description

Species

Halococcus salifodinae DSM 8989 ↗

Extremophile

Yes "halophilic" organism

EC Number

3.4.- ↗

Sequence

Length: 526 amino acids

MVEKPTPGRRSFLKAIGAGTLFGGLSGVASATPGRQPGPKKDEILVGVSASAADMEGAVTQAVPGNAEVVHRNEKLSYVAVKFPSQAADRAKENFIDAITKKDHIKYAEPNTTHEALYQPSDPRFGDQYAPKQVESDRAWDTTLGDSGVTIAVVDTGAQYDHPDLQANYKSNPGRDFADNDSDPYPDAPSSEYHGTHVSGCAAAVVDNGTGVAGQSNSSLINGRALDEGGSGSTADIADAIEWAADQGADIINLSLGGGGYTSTMKNAVSYATDNGSLVIAAAGNNGSSSVSYPAAYSECMAISAVDDNEQLASFSQYGENVELCAPGVDVLSTTTEARGSYERLSGTSMATPVTSGVAGLTLAKWNLTNQELRSHLKNTAEDIGLSANEQGSGQVDALAAVTTDPADGGGGGGGGGGGGETTSGSVDGSLSSYRDGDDYTWSWTYSSPSQVVVELDGPTNADFDLYVNTGTTRNASPNSYDYASRSTNSQETVTIDTPDDSAAMQINVDSYSGSGNYSLTITETQ

Jing Hou et al. (2021) — Biochemical characterization of a low salt-adapted extracellular protease from the extremely halophilic archaeon Halococcus salifodinae

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.02.081 ↗ · Activity - Classical

8 measurements ▼

Database ID

UniParc: UPI000678290D ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Glycerol	15%	100.0	63.2	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	78.4	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100.0	58.6	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Methanol	15%	100.0	46.4	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Acetonitrile	15%	100.0	26.4	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Ethanol	15%	100.0	13.2	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Acetone	15%	100.0	62.6	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Isopropanol	15%	100.0	14.0	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides , Peptides	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Glycerol	15%	100.0	63.2	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100.0	78.4	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100.0	58.6	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Methanol	15%	100.0	46.4	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Acetonitrile	15%	100.0	26.4	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Ethanol	15%	100.0	13.2	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Acetone	15%	100.0	62.6	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 440 nm) in the presence of organic solvent	Isopropanol	15%	100.0	14.0	%	100 mM Tris-HCl buffer, 2 M NaCl	9	45°C	0.5% (w/v) Azocasein	Azo-peptides, Peptides	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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