GH5 endoglucanase (gene celM) from a Tattapan extreme-temperature hot spring metagenomic library

Enzyme Description

Species

Na (Tattapan extreme-temperature hot spring metagenomic library)

Extremophile

Yes likely thermophilic organism (extreme-temperature hot spring), "thermo-halo-tolerant" enzyme

EC Number

3.2.1.4 ↗

Sequence

Length: 304 amino acids

MQRGVNLGNALEAPYEGAWGVILREEYFRLIREAGFDTVRIPIRWSAHANPVPPYTIDERFFRRVDWAIDQALLHGLTVIINVHHYDELMTNPEEHRERFLALWDQIARRYAARPATLLFELLNEPHARLTAPVWNQLLAEALQVVRQTNPTRWVVIGPTQWNHVRSLPSLELPKEDPHIIVTFHYYEPFPFTHQGASWVPGSMVWLGTKWEGREEERQTIVQDLDFAVLWAREQNRPLFLGEFGAYSRADLPSRVRWTEFVARQAEARGIPWCYWEFCSGFGIYDPRTQRWRDQLLWALIPRP

Namrata Joshi et al. (2021) — Biochemical characterization of a novel thermo-halo-tolerant GH5 endoglucanase from a thermal spring metagenome

Biotechnology and Bioengineering · doi:10.1002/bit.27668 ↗ · Activity - Classical

6 measurements ▼

Database ID

UniParc: UPI001AF0C561 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	83	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetonitrile	5%	100	78	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	5%	100	68	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Propanol	5%	100	66	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	5%	100	50	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Butanol	5%	100	26	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	83	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetonitrile	5%	100	78	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	5%	100	68	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Propanol	5%	100	66	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	5%	100	50	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Butanol	5%	100	26	%	20 mM sodium phosphate buffer	6	80°C	0.5% CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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