Subtilisin-like protease from Thermoactinomyces vulgaris CDF

Enzyme Description

Species
Thermoactinomyces vulgaris CDF β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.4.- β†—

Sequence

Length: 389 amino acids
MSPLLKRVLSLAAIFALGTFMSIPTVSNANAETVQKQPQEIIVKFKQNATSSDVSALHAKAKASITSTNKQLGYQVVKVNEGQNMEDVLKQYQSNPNVEYVEPNHEVHAFFTPNDPRYSEQYAPQLVGAEEAWDVTQGSSNVTVAIVDTGVDYNHPDLQGKVIKGKDFVDNDDDPMDENMHGTHCAGIAAALTNNGVGIAGMAPKVKILAERVLDANGSGTLDSVAQGITHAADQGADVISLSLGSPQGASTLENAVNYAASKGAVVVAAAGNESTSAPSYPAYYEKAIAVAATDSNDKIASFSNYGSWVDVAAPGVNILSTVPGGGYQTASGTSMATPLVAGQAALLASQGKSASEIRQAIEGTADQISGTGQYWAHGRVNAAKSVSQ
Yidi Ding et al. (2020) β€” Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF
Frontiers in Microbiology  Β· doi:10.3389/fmicb.2020.605771 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniParc: UPI000673663D β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100.0 115.2 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 40% 100.0 133.6 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Acetone 20% 100.0 93.7 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Acetone 40% 100.0 94.0 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Methanol 20% 100.0 101.0 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Methanol 40% 100.0 86.9 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Ethanol 20% 100.0 94.2 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Ethanol 40% 100.0 81.5 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Isopropanol 20% 100.0 84.3 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (1 hour at 40Β°C), measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 335 nm) in aqueous phase Isopropanol 40% 100.0 67.4 % Incubation: 50 mM Tris–HCl buffer, 10 mM CaCl2, Assay: 50 mM Tris–HCl buffer, 10 mM CaCl2 Incubation: 8, Assay: 8 Incubation: 40Β°C, Assay: 60Β°C 0.25% Azocasein Azo-peptides , Peptides β€” Yes, incubation "with shaking" Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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