Polysaccharide lyase (gene MtAl138) from Microbulbifer thermotolerans DAU221

Enzyme Description

Species

Microbulbifer thermotolerans DAU221 ↗

Extremophile

No but "thermotolerant" organism

EC Number

4.2.2.3 ↗

Sequence

Length: 744 amino acids

MKNSKYTPATTISCAALFSLCMAHAHASIQNSSFENGWDSWTSSGSSAISDIAYSGSKSAKINGSNGYFEQEVTVDANSNYRLTVYVFGEGSVGVESNGNVYSKEVSNSDWELVTVEFNTGSSQTITVFGAYTDGDVRFDDFALEKISSSSTSIENAGFENDWDNWSSSGASAISDISYKGSKSAKVNGSNGRFEQNVAVQPYTDYELTAYILGDGILGVEANGSTYEKSVSNSNWEAETVQFNSGSAQTITIFGEYDGSDGRFDEFSLEEVSSSGSCASTSSLTIVDAYDDGTNDGHGPENVLDGSLADESRWSSQGVKWITLDLGTEKTVEGVDIAWYNGDQRKNYFYIETSIDNINWTTVLNGGQSSGTSSDMEFYDVADTTARYVRLTGSGNSYDSWNSILEFDVIGCGDGSEESSSSSSSSSSSSSSSSSSSSSSSSSSGGGSSSSSSSSSSSGGFSNLDPSLPPGSNFELEDWYLSIPTDDDGNGKADSIKEGALSGGYENSSYFYTASDGGMVFRCPIDGYKTSSGTSYTRTELREMLRRGDTSIDTQGVNKNNWVFGSAPAEAREAAGGVDGVLRATLAVNHVTTTGDSSQVGRVIIGQIHANDDEPLRIYYRKLPDNTKGSIYIAHEPNGGSDQWYEMIGSRSDDASDPADGIALNEIFSYEVKVVGNTLTVTISRDGKEDVVQEVDMSDSGYDTEDQYQYFKAGVYNQNNTGDDDDYVQATFYSLENSHDGYDY

Hae-Rin Jeong et al. (2021) — Characterization of an organic solvent-tolerant polysaccharide lyase from Microbulbifer thermotolerans DAU221

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.12.138 ↗ · Activity - Classical

22 measurements ▼

Database ID

UniProt: A0A7D5KCK1 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (22 measurements)

22 measurements — page 1 of 2

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	9	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	n-Hexane	20%	100	205	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Toluene	20%	100	156	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Benzene	20%	100	358	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isoamyl Alcohol	20%	100	78	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	1-Butanol	20%	100	33	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isopropanol	20%	100	79	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetone	20%	100	0	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Ethanol	20%	100	25	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetonitrile	20%	100	14	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Methanol	20%	100	114	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	12	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	n-Hexane	10%	100	169	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Toluene	10%	100	118	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Benzene	10%	100	130	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isoamyl Alcohol	10%	100	79	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	1-Butanol	10%	100	16	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isopropanol	10%	100	136	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetone	10%	100	3	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Ethanol	10%	100	38	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	9	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	n-Hexane	20%	100	205	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Toluene	20%	100	156	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Benzene	20%	100	358	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isoamyl Alcohol	20%	100	78	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	1-Butanol	20%	100	33	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isopropanol	20%	100	79	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetone	20%	100	0	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Ethanol	20%	100	25	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetonitrile	20%	100	14	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Methanol	20%	100	114	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	12	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	n-Hexane	10%	100	169	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Toluene	10%	100	118	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Benzene	10%	100	130	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isoamyl Alcohol	10%	100	79	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	1-Butanol	10%	100	16	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Isopropanol	10%	100	136	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetone	10%	100	3	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Ethanol	10%	100	38	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Acetonitrile	10%	100	29	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (Δ4.5-unsaturated oligoalginate product absorbance measurement, 235 nm) in the presence of organic solvent	Methanol	10%	100	127	%	20 mM sodium phosphate buffer	7.4	45°C	0.2% (w/v) Alginate	Δ4,5-unsaturated oligoalginate	—	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

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Structure

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