Lipase (gene LipI.3_KE38) from Pseudomonas fluorescence KE38
Enzyme Description
Extremophile
Yes
"psychro-tolerant" organism, "cold-active" enzyme
EC Number
Sequence
MGVYDYKNFSTADSKALFTDAMAITLYSYHNLDNGFATGYQHNGLGLGLPATLVTALLGGTNSQGVIPGIPWNPDSEKAALDAVQKAGWTPITASQLGYDGKVDAHGTFFGEKAGYNTAQVEILGKYDAQGHLTEIGIAFRGTSGPRETLIGDSIGDVINDLLAALGPKDYAKNYVGEAFGNLLGDVVAFAQANGLSGKDVLVSGHSLGGLAVNSLADLSTDTWGGFFKDSNYIAYASPTQSATDNVLNVGYENDPVFRALDGSSFNLSSVGVHDAPKESATDNIVSFNDHYASTAWNVLPYSILNIPTWISHLPTAYGDGMNRVIESKYYDLTSKDSTIVVANLSDPARANTWVQDLNRNAETYKGSTFIIGSDANDLIQGGNGNDYLEGRDGNDTFRDGGGYSILLGGKGNNVLDLQQSVKAVEFAHDGAGTLYLRDANGGISITRDIGSIVSKEPGFLWGLFKDDVTHSVTANGLAVGNHLTAYASSVKGGGGADTLKAQATGDWLFGLDGNDHLIGGKGNDVFVGGAGNDVMESGGGVDTFLFSGSFGQDRVVGYQANDKLVFLGVQGVVANDDFRAHATAVGHDTVLTFGGDSVTLVGVGLDSLAGTGIVIA
Fulya KarakaΕ et al. (2020)
β Gene cloning, heterologous expression, and partial characterization of a novel cold-adapted subfamily I.3 lipase from Pseudomonas fluorescence KE38
Scientific Reports
Β· doi:10.1038/s41598-020-79199-w β
Β· Activity - Classical
▼
Database ID
UniProt: A0A7T6AN61 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | Acetone | 30% | 100 | 90 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | Acetonitrile | 30% | 100 | 135 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | Methanol | 30% | 100 | 100 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | Ethanol | 30% | 100 | 125 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | Isopropanol | 30% | 100 | 10 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | n-Hexane | 30% | 100 | 65 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by titration (pH-stat) in the presence of organic solvent | Ethyl Acetate | 30% | 100 | 30 | % | 50 mM Tris-HCl buffer incubation | 8.5 | 25Β°C | 5% Olive oil | free fatty acids , glycerol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.