Keratinase from Bacillus sp. CSK2
Enzyme Description
Sequence
GCTLDEVAQGIGEVADSGAKVIRIRLGAPKGGIALPQAVRYAWNKGSVIVAAAGNVGNTKANYPAYNSEVIAVASTDQSDRKCSFSTYGSWVDVAAPGSNIYSTYKGSTYQSLSGTSMATPHVAGVAALLANQGYSNTQIRQIIESTTDKISGTGTY
Nonso E. Nnolim et al. (2020)
β Bacillus sp. CSK2 produced thermostable alkaline keratinase using agro-wastes: keratinolytic enzyme characterization
BMC Biotechnology
Β· doi:10.1186/s12896-020-00659-2 β
Β· Stability - Incubation
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Database ID
UniParc: UPI0018A42ACA β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Bacillus sp. CSK2
Experimental Data (3 measurements)
3 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 37Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 595 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 1% | 100 | 114 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 37Β°C, Assay: 60Β°C | 5 g/l Keratin azure | Azo-peptides , Peptides | β | Assay: 220 rpm | Water-incubated control (in %), assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (1 hour at 37Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 595 nm) in aqueous phase | Acetonitrile | 1% | 100 | 61 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 37Β°C, Assay: 60Β°C | 5 g/l Keratin azure | Azo-peptides , Peptides | β | Assay: 220 rpm | Water-incubated control (in %), assay in aqueous conditions |
| Stability - Incubation | Activity after incubation (1 hour at 37Β°C), measured by absorbance spectrophotometry (colorimetric assay, Azo-peptides absorbance measurement, 595 nm) in aqueous phase | Isopropanol | 1% | 100 | 79 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 37Β°C, Assay: 60Β°C | 5 g/l Keratin azure | Azo-peptides , Peptides | β | Assay: 220 rpm | Water-incubated control (in %), assay in aqueous conditions |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.