Lipase BTL2 from Geobacillus thermocatenulatus

Enzyme Description

Species
Geobacillus thermocatenulatus β†—
Extremophile
Yes thermophilic organism
EC Number
3.1.1.3 β†—

Sequence

Length: 417 amino acids
MMKGCRVMVVLLGLWFVFGLSVPGGRTEAASPRANDAPIVLLHGFTGWGREEMLGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSIPGAEKLNQWVQASPNTYYLSFSTERTHRGALTGNYYPELGMNAFSAVVCAPFLGSYRNEALGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTCNVDHLEVIGVDPNPSFDIRAFYLRLAEQLASLRP
Jun Zhang et al. (2020) β€” High-efficiency expression of the thermophilic lipase from Geobacillus thermocatenulatus in Escherichia coli and its application in the enzymatic hydrolysis of rapeseed oil
3 Biotech  Β· doi:10.1007/s13205-020-02517-6 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: Q59260 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Methanol 1% 100.0 72.74 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Ethanol 1% 100.0 107.29 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isopropanol 1% 100.0 47.36 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent 1-Butanol 1% 100.0 102.93 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Isoamyl Alcohol 1% 100.0 54.71 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Benzene 1% 100.0 72.24 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Toluene 1% 100.0 81.29 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Styrene 1% 100.0 79.28 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent n-Hexane 1% 100.0 117.9 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent n-Heptane 1% 100.0 76.44 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Acetone 1% 100.0 82.29 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent Furfural 1% 100.0 67.57 % 50 mM Tris-HCL buffer 8 60Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Classical aqueous control (in %) | Uncertainty about whether it might be an incubation assay instead

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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