Esterase (gene EstSHJ2) from Chromohalobacter canadensis

Enzyme Description

Species

Chromohalobacter canadensis ↗

Extremophile

Yes "halophilic" organism

EC Number

3.1.1.1 ↗

Sequence

Length: 213 amino acids

MLVAPVANILYLHGFNSGAASPKARLIAATCERLASEDVAPPRCVTPQLPHRPDEALACAERELHALGNNTLLIGSSMGGFLATCLAERFGVRAVLINPAVRPARLVTQWMGAPLENPYTHERFTIDERHGEALEAMTPAAVIDPGRYQVLLGSADETLNCRDALTFYAGASMLVHPSGDHGFSALADYLPAIFAHGGWTLPARSYTRSDEST

Mou Wang et al. (2020) — Characterization of a novel halotolerant esterase from Chromohalobacter canadensis isolated from salt well mine

3 Biotech · doi:10.1007/s13205-020-02420-0 ↗ · Activity - Classical

20 measurements ▼

Database ID

UniProt: A0A2I4SAV9 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	95	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	93	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	100	58	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	102	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethyl Acetate	30%	100	101	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethyl Acetate	10%	100	105	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	30%	100	97	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	10%	100	103	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	100	64	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	10%	100	107	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	30%	100	78	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100	103	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	30%	100	156	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	10%	100	110	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	30%	100	61	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	10%	100	98	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	30%	100	126	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	10%	100	106	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	30%	100	135	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	95	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	56	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	93	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	100	58	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	102	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethyl Acetate	30%	100	101	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethyl Acetate	10%	100	105	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	30%	100	97	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	1-Propanol	10%	100	103	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	100	64	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	10%	100	107	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	30%	100	78	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100	103	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	30%	100	156	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	10%	100	110	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	30%	100	61	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetonitrile	10%	100	98	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	30%	100	126	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Benzene	10%	100	106	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Toluene	30%	100	135	%	35 mM Tris-HCl buffer, 20% isopropanol	8	37°C	2 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %) \| No completely clear it is an activity assay (might be an incubation)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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