Cysteine endopeptidase EP-B 2-like from Zea mays

Enzyme Description

Species
Zea mays β†—
Extremophile
No
EC Number
3.4.- β†—

Sequence

Length: 368 amino acids
MAQVAKTLLLVALVVVSAVELCRAIEFDERDLASDEALWDLYERWQTHHRVHRHHGEKGRRFGTFKENARFIHAHNKRGDRPYRLRLNRFGDMGREEFRSGFADSRINDLRREPTAAPAVPGFMYDDATDLPRSVDWRQKGAVTAVKNQGRCGSCWAFSTVVAVEGINAIRTGSLVSLSEQELIDCDTDENGCQGGLMENAFEFIKSHGGITTESAYPYHASNGTCDGARARRGRVVAIDGHQAVPAGSEDALAKAVAHQPVSVAIDAGGQALQFYSEGVFTGDCGTDLDHGVAAVGYGVSDDGTPYWIVKNSWGPSWGEGGYIRMQRGTGNGGLCGIAMEASFPIKTSPNPSRKPRRALITRDASSQ
Wenjing Zhang et al. (2020) β€” Characterization of a recombinant zein-degrading protease from Zea mays by Pichia pastoris and its effects on enzymatic hydrolysis of corn starch
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.08.237 β†—  Β· Activity - Classical
8 measurements ▼
Database ID
NCBI: NP_001130571.2 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris X33

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 15% 100 17 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 30% 100 5 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Methanol 15% 100 46 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Methanol 30% 100 16 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Acetone 15% 100 33 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Acetone 30% 100 23 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15% 100 13 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, azo-peptide absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100 10 % 100 mM citrate -Na2HPO4 buffer 5 40Β°C 2.5 mg/ml Azocasein Azo-peptides , Peptides β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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