Laccase (gene lac2) from Cerrena unicolor CGMCC 5.101

Enzyme Description

Species

Cerrena unicolor CGMCC 5.101 ↗

Extremophile

No but "thermotolerant" enzyme

EC Number

1.10.3.2 ↗

Sequence

Length: 532 amino acids

MGVGLLSLLPTTILAVGVSAAIGPISDLNIVNKNITADGFERPTVLAGGTFPGPLIIGNKGDNFQLNVIDQLVDDRMLTATSMHWHGLFQNGTNWADGPPFVTQCPIVANDSFLYNFTVPGQAGTFWYHSHLETQYCDGLRGPLVVYDPNDPNKDLYDVDNETTVITLADWYHVLAQAVVGAAIPDSTLINGLGRSPGNLDANLSVITVESSKRYRFRLISISCDPNFISSIDNHNMTVIETDGVNTKSLDVDSIQIFAGQRYSFVLEANQSVANYWIRAQPNNGMDTSFSGGLNSAILRYSGAPDEGPQTNQTPSTAPLLETNLHPLESLPVPGSPVPGGADLVHNFTLAFSSGRFSIDGTSWIIPSNFTLPVLLQILSGQTDAHQLLPQGSILDVGFDQVVELVFPPGTAIEGSHPFHLHGHNFWVVRGAGSDQYNFDDPIVRDVVSIGNNSDLVTIRYVTDNAGPWFLHCHIDWHLQTGFGIVLAEGINQTAAHNPVPPAWDQLCSKYSATLPTTAAVAVATAAQTSAS

Yanhua Yao et al. (2020) — A highly thermotolerant laccase produced by Cerrena unicolor strain CGMCC 5.1011 for complete and stable malachite green decolorization

AMB Express · doi:10.1186/s13568-020-01118-z ↗ · Activity - Classical

12 measurements ▼

Database ID

UniProt: A0A7U3NTD2 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli Top10

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	94	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	25%	100	84	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	102	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	25%	100	82	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	88	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	25%	100	56	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	100	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	25%	100	63	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetonitrile	10%	100	94	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetonitrile	25%	100	51	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	95	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	71	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	10%	100	94	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Methanol	25%	100	84	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	10%	100	102	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Ethanol	25%	100	82	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	10%	100	88	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetone	25%	100	56	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	10%	100	100	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Isopropanol	25%	100	63	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetonitrile	10%	100	94	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Acetonitrile	25%	100	51	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	95	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	71	%	Citrate–phosphate buffer	3	55°C	? mM ABTS	ABTS radical cation	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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