Esterase (gene CrmE10) from Croceicoccus marinus E4A9T

Enzyme Description

Species

Extremophile

EC Number

3.1.1.1 ↗

Sequence

Length: 205 amino acids

MADGEAAGQQADAVMPTGPAIDVLAFGDSLFAGYRLDRDESYPARLQAALRERGLNVNVTNAGVSGDTTAAGLQRIDFVLDSMAGEPDLVLLELGANDMLRGLPAEEARRNLDTILQRLDQRDIPVMVYGMRAAPNLGGDYGRSFDSIFPDLADKYDAELVPFFIEPLIFDRSLVQQDQLHPTAQGVDAMVEQTVEQVEDRIDDL

Zhengyang Li et al. (2020) — Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases

Biotechnology for Biofuels · doi:10.1186/s13068-020-01742-8 ↗ · Activity - Classical

8 measurements ▼

Database ID

UniProt: A0A1Z1F9L9 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli DH5alpha

Experimental Data (8 measurements)

8 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	15%	100	23	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	15%	100	0	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100	59	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	22	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	104	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Glycerol	15%	100	155	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	15%	100	75	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100	77	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	15%	100	23	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetonitrile	15%	100	0	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100	59	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	22	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	104	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Glycerol	15%	100	155	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	15%	100	75	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100	77	%	100 mM Tris–HCl buffer	7.5	20°C	1 mM p-Nitrophenyl hexanoate	Hexanoic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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