Cellulase (gene MaCel) from Mahella australiensis 50-1 BON

Enzyme Description

Species
Mahella australiensis 50-1 BON β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.4 β†—

Sequence

Length: 426 amino acids
MNKRKMLAFMIAIVLILSSLMTSTIVVRKTEAAASSIVASHGMLKVVGNKVCDASGKPIQLKGMSLFWSQWSTPFWNADLVNNLASTWNATLIRAAMGVDEGGYISNPQVEKQKVITVVDAAINAGIYVIIDWHSHDIHTSEAVAFFGEMAQRYKNTPNVIFEIFNEPDYESWAQVKTYSTQVINTIRSTGAQNLILVGSPTWSQDVDVAANDPITGFSNIAYTLHFYAGTHKQYLRDKAITAMSKGLALFVSEWGTCDASGNGNLDLTESQRWIDFMNQYQISWANWSMNDKAETASALVVGASTTGPWPDSQLTESGKFVKAKIMENTPVPPSGGTTPPGEYTGIGLPFTHDGAGEYYWKTNKLSTDPNDWSHYINSWNLDLLEINGKDYTNVWVAQHQIAPSSDGYWYIHYKGSASSAHMEMK
Tong Zhu et al. (2020) β€” Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2020.05.021 β†—  Β· Activity - Classical
16 measurements ▼
Database ID
UniProt: F4A377 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 5% 100 100 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 10% 100 92 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 15% 100 87 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 20% 100 78 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 5% 100 97 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 10% 100 88 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 15% 100 78 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 20% 100 72 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 5% 100 98 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 10% 100 94 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 15% 100 91 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 20% 100 80 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 5% 100 95 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 10% 100 81 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 15% 100 75 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 20% 100 59 % 50 mM Tris-HCl buffer, 0.5 MM CoCl2 7.5 65Β°C 0.9% (w/v) CMC (Carboxymethylcellulose sodium) Reducing sugars β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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