GH10 xylanase (gene xynM1) from a hot spring metagenomic library

Enzyme Description

Species

Na (hot spring metagenomic library)

Extremophile

Yes likely "thermophilic" organism (from high temperature hot spring), "thermophilic" enzyme

EC Number

3.2.1.8 ↗

Sequence

Length: 349 amino acids

MLSFIIREREVDELRNQVSRLRTEIEQLRGAVPLLKTLAEKHGVYIGAAVGADQLIIKEYAEILSREFNILTTENALKFERVHPERNVYSFAEADVIISFAEASGMRVRGHTLVWHYQLPEWITRGNYSREEWISILHDHITTVVSHYRGRVYAWDVVNEAIDDDGSLRKTIWLENIGPEYIELAFKWAHEADPQALLFYNDYGAEGLGVKSDAVYNLVKNLLEKGVPIHGVGLQMHVSTEYYPAPQDIAANIKRLSDLGLEIHITEMDVCVRLPAKSADLVRQAEIYRDVLMVCLSSEKFTAFVMWGFTDRYSWIPGFFSGYGSALIFDENYHPKPAYYYILKSLMQP

Namrata Joshi et al. (2020) — Characterization of a novel xylanase from an extreme temperature hot spring metagenome for xylooligosaccharide production

Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10562-7 ↗ · Activity - Classical

6 measurements ▼

Database ID

UniParc: UPI00159A909E ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	92	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	1%	100	97	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	1%	100	63	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Propanol	1%	100	81	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Butanol	1%	100	44	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetonitrile	1%	100	97	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	92	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Methanol	1%	100	97	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Ethanol	1%	100	63	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Propanol	1%	100	81	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	1-Butanol	1%	100	44	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent	Acetonitrile	1%	100	97	%	50 mM sodium phosphate buffer	7	80°C	0.2% Soluble birchwood xylan	Reducing sugars	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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