Nitrite reductase (gene NirKHrs) from Halorussus sp. YCN54
Enzyme Description
Sequence
MPATSRRIVLQGLGIGGAASLAGCAGQSAPSATDTTTQTTEETDTETDADRVAADPTDIPDPIDRSEPAEVDVTLRAEEVTAEVEDGVTFDYMTYNGQAPGPFIRVRKGDTVNLTFENPTENDLPHNVDFHAAAGPGGGAEATMTAPGETARLKFKATYPGAYIYHCAVPNMDMHISAGMFGIILVEPEDGLPEVDHELYLGQHELYTDKRAGEEGHHGFDMSAMAREEPSYVVMNAEKYAITPDGYGAMGVETGDTARVYFVTGGPNLTSNFHPIGNVFEELWPEGSLSSDPQTHIQTKPVAPGSTTVATMGFEVPGPYKLVDHALSRVARKGCMAVVAAEGEENPEVFDPEPGE
Jing Hou et al. (2020)
β Characterization of a novel Cu-containing dissimilatory nitrite reductase from the haloarchaeon Halorussus sp. YCN54
Extremophiles
Β· doi:10.1007/s00792-020-01164-2 β
Β· Activity - Classical
▼
Database ID
UniParc: UPI0020A19335 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host archaeon Haloferax volcanii strain H1424
Experimental Data (14 measurements)
14 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Glycerol | 15% | 100.0 | 106.9 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 15% | 100.0 | 110.0 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 15% | 100.0 | 140.9 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Methanol | 15% | 100.0 | 77.3 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Acetonitrile | 15% | 100.0 | 83.5 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Ethanol | 15% | 100.0 | 67.0 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Acetone | 15% | 100.0 | 59.4 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Glycerol | 30% | 100.0 | 136.9 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 115.3 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Dimethylformamide (DMF) | 30% | 100.0 | 157.6 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Methanol | 30% | 100.0 | 56.6 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Acetonitrile | 30% | 100.0 | 81.5 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Ethanol | 30% | 100.0 | 24.4 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, diazo-coupling assay with decrease in nitrite absorbance measurement) in the presence of organic solvent | Acetone | 30% | 100.0 | 52.5 | % | 100 mM phosphate buffer, 2M NaCl, 16 mM sodium dithionite (electron donor), 3 mM methyl viologen (electron carrier) | 7 | 55Β°C | 5 mM Sodium nitrite | Reduced nitrogen species | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.