GH39 Ξ²-glucosidase (gene cmgh1) from Croceicoccus marinus E4A9T

Enzyme Description

Species
Croceicoccus marinus E4A9T β†—
Extremophile
No
EC Number
3.2.1.21 β†—

Sequence

Length: 485 amino acids
MTSDLIAKLSVNAGEPIGNMRQLHGTSGIPAPAPGTDSVPDILDVWRNAQVTLVRSYDWVSRLDTIDNPTSLFPDWSADPSDPASYNFAATDTWVGQTRSIGANILFTIASEIPANKQPARDLAKYEQVVENIVRHYVCGWGDGFENAVSHWEFGDQPDFGKLHFSGTPDQFYEMYAAAARAVKRVDPALKVGGPCVAFPLNEGPFREGFLDYVKQQSVPLDFLSWMWYGDNSRDPMDFRTIAAEVRAIVDKYGFTDTELLLSYWSMTGIPTAKFEDFDNAAFLAAAAIYMQDSEVDKAIFFRADTGADFHYNFTDPAGIFEDDGSQNARTGAFQLVGQTLATTERLAITGGDDNGFAALAGRTADGDTIRILISNYAIPDMYLTARDRDVFEFQVPIGDQKTDMSLNVPPRRVDARSTGYSGYTLEIGHLPWGDGPHRVVRYRADRDHKGEMLDSHEGRGSSVTVQNKLAVSGVELIEITRVSS
Yanfang Shen et al. (2019) β€” Structural and Functional Insights Into CmGH1, a Novel GH39 Family Ξ²-Glucosidase From Deep-Sea Bacterium
Frontiers in Microbiology  Β· doi:10.3389/fmicb.2019.02922 β†—  Β· Activity - Classical
16 measurements ▼
Database ID
UniProt: A0A1Z1FG87 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3) pLysS

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 5% 100 92 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 15% 100 63 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 5% 100 93 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 15% 100 55 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 5% 100 101 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 15% 100 66 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 5% 100 97 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 15% 100 92 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 5% 100 101 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 15% 100 65 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 5% 100 80 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Glycerol 15% 100 50 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 5% 100 98 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 15% 100 66 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100 101 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15% 100 88 % 100 mM Tris–HCl buffer 7.5 20Β°C-60Β°C 1 mM p-Nitrophenyl Ξ²-D-glucopyranoside p-Nitrophenol , Ξ²-D-glucose β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*