Ene-reductase (gene FOYE-1) from Ferrovum sp. JA12

Enzyme Description

Species

Ferrovum sp. JA12 ↗

Extremophile

Yes "acidophilic" organism

EC Number

1.3.1.31 ↗

Sequence

Length: 375 amino acids

MGHHHHHHHHHHSSGHIEGRHMSLLFSPYQLGSLSLANRLVIAPMCQYSAVDGIAQDWHLMHLGRLAISGAGLVIVEATGVNPEGRITPFCLGLYNDEQEAALGRIVAFAREFGQAKMAIQLAHAGRKASTRRPWDPGSPYSPEEGGWQTWAPSAIKFYEESLTPHPMSIEDLETVKQDFVNSAIRAERAGFKAIELHGAHGYLIHQFLSPLSNQRQDQYGGSLENRMRYPLEILSAVKHALSAEMVVGMRISAVDWAPGGLTIEESITFSQECEKRGAGFIHVSTGGLVAHQQIPVGPGYQVEHAQAIKQNVNIPTMAVGLITHSAQAETILKSEQADMIAIARAALKNPHWPWTAALELGDKPFAPPQYQRAR

Dirk Tischler et al. (2020) — Asymmetric Reduction of ( R )-Carvone through a Thermostable and Organic-Solvent-Tolerant Ene-Reductase

ChemBioChem · doi:10.1002/cbic.201900599 ↗ · Activity - Classical

63 measurements ▼

Database ID

UniParc: UPI00070287B1 ↗

Sequence Annotation

Explicit - Provided (hist-tag included)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (63 measurements)

63 measurements — page 1 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	40%	100	15	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	30%	100	28	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	40%	100	17	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	50%	100	10	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	108	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	92	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	100	18	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	50%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	67	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	100	49	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	40%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	64	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	20%	100	41	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	20%	100	41	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100	43	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	20%	100	22	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	40%	100	13	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	40%	100	15	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	30%	100	28	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	40%	100	17	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	50%	100	10	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	108	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	92	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	100	18	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	50%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	67	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	100	49	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	40%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	64	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	20%	100	41	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	20%	100	41	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100	43	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	20%	100	22	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	40%	100	13	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	63	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	20%	100	33	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	40%	100	6	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	49	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	22	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	3	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100	20	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	20%	100	6	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	40%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 1 mM BNAH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	50%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	20%	100	120	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	30%	100	112	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	40%	100	47	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	50%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100	98	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	20%	100	97	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	30%	100	88	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	40%	100	62	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	50%	100	30	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100	120	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	20%	100	114	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	30%	100	96	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	40%	100	50	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100	104	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100	110	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	20%	100	108	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	30%	100	86	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	40%	100	67	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	50%	100	31	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	60%	100	16	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	103	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	86	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	48	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	14	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100	0	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100	63	%	50 mM phosphate buffer	7.1	22°C	1 mM N-Methylmaleimide	N-Methylsuccinimide	FMN, electron donor: 0.2 mM NADPH	—	Classical aqueous control (in %)

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Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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