Protease from Bacillus cereus ATCC 4342

Enzyme Description

Species

Extremophile

EC Number

3.4.- ↗

Sequence

Length: 316 amino acids

VTGTNKVGTGKGVLGDTKSLNTTLSGSSYYLQDNTRGATIFTYDAKNRSTLPGTLWADADNVFNAAYDAAAVDAHYYAGKTYDYYKATFNRNSINDAGAPLKSTVHYGSNYNNAFWNGSQMVYGDGDGVTFTSLSGGIDVIGHELTHAVTENSSNLIYQNESGALNEAISDIFGTLVEFYDNRNPDWEIGEDIYTPGKAGDALRSMSDPTKYGDPDHYSKRYTGSSDNGGVHTNSGIINKQAYLLANGGTHYGVTVIGIGKDKLGAIYYRANTQYFTQSTTFSQARAGAVQAAADLYGANSAEVAAVKQSFSAVGV

Tolbert Osire et al. (2019) — Lys–Arg mutation improved the thermostability of Bacillus cereus neutral protease through increased residue interactions

World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-019-2751-5 ↗ · Activity - Classical

7 measurements ▼

Database ID

—

Sequence Annotation

Explicit - Provided (249 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21

Experimental Data (7 measurements)

7 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Benzene	10%	100.0	61.0	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	10%	100.0	89.28	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	84.63	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	10%	100.0	94.32	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	10%	100.0	112.91	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	10%	100.0	91.61	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	94.7	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Benzene	10%	100.0	61.0	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Ethanol	10%	100.0	89.28	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	84.63	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	10%	100.0	94.32	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	10%	100.0	112.91	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	10%	100.0	91.61	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reactant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	94.7	%	Tris-HCl buffer	7.4	37°C	1% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*