Endoglucanase (gene CKT3eng) from Haloarcula sp. strain CKT3

Enzyme Description

Species

Haloarcula sp. strain CKT3 ↗

Extremophile

Yes "extreme halophilic" organism

EC Number

3.2.1.4 ↗

Sequence

Length: 352 amino acids

MDTRRREFLESLLTTPGPSGYEADSQRVWLDYVSEFADEVRTDDYGNAVAKVEGGNTIVALAGHGDEIGFIVRDFTDDGFVKLGRIGGSDKTVSRGQHVTIHTADGPVSGVVGQTAIHLRDSDDDSVPEISEQYVDVGAADGDEVESLVDRGDPVTFVQTLSELENGRLAARGMDNRIGIWAAAEGLHRAAESDPDATVYAVSTVQEEVGVQGAKMVGFDLAPDVAIAADVTHATDAPGSPGKAATGVDLGEGPVVARGSANYPVAVDALRETSEAEDIDIQLQAAGTRTGTDADAFYTSRGGIPSVNVGLPNRYMHTPVEVIDPDDLDALADLLAGFAVRAADRAPFSVDV

Haktan Şafak et al. (2020) — Molecular and biochemical characterization of a recombinant endoglucanase rCKT3eng, from an extreme halophilic Haloarcula sp. strain CKT3

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.10.161 ↗ · Stability - Incubation

6 measurements ▼

Database ID

UniParc: UPI001317CE90 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	25%	100.0	70.8	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100.0	75.5	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	12.5%	100.0	73.7	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	10%	100.0	75.9	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	10%	100.0	89.9	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Toluene	10%	100.0	87.9	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions

Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	25%	100.0	70.8	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100.0	75.5	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	12.5%	100.0	73.7	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	10%	100.0	75.9	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Benzene	10%	100.0	89.9	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions
Stability - Incubation	Activity after incubation (24 hours at 50°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Toluene	10%	100.0	87.9	%	50 mM Tris-HCl buffer, 3 M NaCl buffer, 50 mM Tris-HCl buffer, 3.4 M NaCl assay	Incubation: 7, Assay: 8	Incubation: 50°C, Assay: 50°C	0.5% (w/v) CMC (Carboxymethylcellulose sodium)	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about control, uncertainty about incubation conditions

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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