Sucrose phosphorylase from Thermobacillus sp. ZCTH02-B1
Enzyme Description
Species
Extremophile
Yes
"thermophilic" organism
EC Number
Sequence
MKLENRVQLITYPDSLGGNLAALREVLRRHFPGLFLGGIHILPPFPSSGDRGFAPLDYGMIDPAFGTWEDIAQLGEEYGVMLDVMVNHISRRSPQFMDFLKQGRSSPYADMFLTLDKIWPDGRPVQADIDKMFLRRKLPYSAFTVGDGREELVWTTFGKTDPSEQIDLDVRSPLARHYLSGIFQRFKANRVNMVRLDAVGYVIKKPGTSCFFVEPDIYEFLDWVADQAKEHDIALLPEVHAHYSIQYKLAERGFWIYDFILPFMVLDALVNRSSAMLLRYLRTRPANQFTMLDCHDGIPVKPDLDDLVDTAQARRVVDLCLARGANLSYVISDRHKSPDGFDVHQIRCTYYDALGRNDDAYLAARAIQLFVPGIPQIYYVGLLAGENDPARAEATGDGREINRHNYTLEEIEQAVRRPVVQRLLKLIRFRNEHEAFQGSFRVCESEDSKIRLEWEKDAIRCALHVDLDTYRSIITCTDGQGKEAQWVV
Yan Li et al. (2019)
β Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-Ξ±-d-glucopyranosyl-l- ascorbic acid
Journal of Biotechnology
Β· doi:10.1016/j.jbiotec.2019.08.018 β
Β· Activity - Classical
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Database ID
UniParc: UPI000B55A2E2 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100 | 136 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 132 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 35% | 100 | 125 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40% | 100 | 121 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 45% | 100 | 71 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 39 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 55% | 100 | 9 | % | 50 mM sodium phosphate buffer | 7 | 55Β°C | 150 mM Sucrose | fructose , Glucose | β | β | Classical aqueous control (in %) | EC number given for hydrolysis reaction, 2.4.1.7 for the EC number corresponding to the transferase reaction |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.