Lipase (gene Lip479) from a Taptapani hot spring sediments metagenomic library

Enzyme Description

Species
Na (Taptapani hot spring sediment metagenomic library)
Extremophile
Yes "thermostable" enzyme, organism from hot spring sediment
EC Number
3.1.1.3 β†—

Sequence

Length: 392 amino acids
RTEAASLRANDAPIVLLHGFTGWGREEMFGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRIHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLEAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSVSGAEKLNQWVQASPNTYYLSFSTERTYRGALTGNHYPELGMNAFSAVVCAPFLGSYRNPTLGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTYNVDHLEIIGVDPNPSFDIRAFYLRLAEQLASLRP
Rajesh Kumar Sahoo et al. (2019) β€” Characterization of novel metagenomic–derived lipase from Indian hot spring
International Microbiology  Β· doi:10.1007/s10123-019-00095-z β†—  Β· Stability - Incubation
9 measurements ▼
Database ID
UniParc: UPI000676B753 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli DH5alpha

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Glycerol 25% 100.0 62.88 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100.0 114.73 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dimethylformamide (DMF) 25% 100.0 122.34 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 25% 100.0 100.59 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 25% 100.0 77.61 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Acetone 25% 100.0 126.3 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Isopropanol 25% 100.0 63.24 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Dichloromethane 25% 100.0 109.17 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at ?Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Chloroform 25% 100.0 18.34 % Incubation: ?, Assay: 100 mM Tris-HCl buffer Incubation: ?, Assay: 8 ? ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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