NAD(P)-dependant isocitrate dehydrogenase from Aspergillus fumigatus Af293

Enzyme Description

Species

Extremophile

EC Number

1.1.1.41 ↗

Sequence

Length: 385 amino acids

MISARNFVAPARQCLRTTRVSSGFASHLSQLRGYASAADDRVAKFKGQKGPDGKYTVTLIEGDGIGPEIAQSVKDIFAAAKAPIKWEPVDVTPILKDGKTTIPEEAIKSVQKNYVALKGPLATPVGKGHVSLNLTLRRTFNLFANLRPCRSVAGYKTPYDNVDTVLIRENTEGEYSGIEHVVVDGVVQSIKLITREASERVLRFAFQYARSVNKKKVRVVHKATIMKMSDGLFLNTAREIAKEFPDIEFDAELLDNSCLKIVTDPTPYNDKVLVMPNLYGDILSDMCAGLIGGLGLTPSGNIGDECSIFEAVHGSAPDIAGKGLANPTALLLSSIMMLQHMGLHEHAARIQKATFDTLAEGKTLTGDLGGKAKTHEYAEAICKRL

Wei Jiang et al. (2019) — Coenzyme Binding Site Analysis of an Isopropanol Dehydrogenase with Wide Substrate Spectrum and Excellent Organic Solvent Tolerance

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-019-03091-1 ↗ · Activity - Classical

12 measurements ▼

Database ID

UniProt: E9QYY1 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100.0	5.9	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	30%	100.0	12.1	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100.0	14.3	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	30%	100.0	19.6	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100.0	68.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	30%	100.0	100.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	83.9	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	121.7	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100.0	47.5	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	30%	100.0	50.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetophenone	10%	100.0	46.2	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetophenone	30%	100.0	12.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol

Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	10%	100.0	5.9	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Methanol	30%	100.0	12.1	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	10%	100.0	14.3	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Ethanol	30%	100.0	19.6	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	10%	100.0	68.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Isopropanol	30%	100.0	100.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	10%	100.0	83.9	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetonitrile	30%	100.0	121.7	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	10%	100.0	47.5	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetone	30%	100.0	50.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetophenone	10%	100.0	46.2	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol
Activity - Classical	Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent	Acetophenone	30%	100.0	12.0	%	200 mM PBS buffer	6	40°C	? mM 1-Butanol	Butyraldehyde	10 µM NADP+	—	Control (in %) \| Reference : activity in 30 % isopropanol

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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