Laccase from Cohnella sp. A01

Enzyme Description

Species
Cohnella sp. A01 β†—
Extremophile
Yes extremophilic organism
EC Number
1.10.3.2 β†—

Sequence

Length: 534 amino acids
MTDNTDNMEGMDHSNMQMGQSDTSKSDPNQMNHMDHSNMPGMNKVQNMDEPAGSPQVLTGTKFTLTAKESHLMVNETNMRTAMTFNGTVPGPQIRVKLGDEVEITLKNELDEPTTIHWHGLPVPNNMDGIPGVTMNAVQPGKSFTYKFKATVPGTYWYHSHQEGVVQVDKGLYGSFIVEDPKEKKADRDYTIVLDEWMEDGEMDHSSVNMSTMDMGSMDHSNMGSMNHSKMNMGSTGTGRMMSDAEMMPMMYTIFTANGKSGSSIEPLTVKEGETVRIRLINAGFLSHPLYLQGHEFKIISSDGQPINNPPLVSGQLLNIAPGERYDIEFVADNPGVWLLGEQSKNPGASTLTIPIVYEGAKGNYTPDNEQRPVINMTRYGEAAKAQFSLNDKFDLEYTMDLNTATADGHFIYTINGKTFPDTDPIKVKEGDLIKVKLINNSPEDLHPMHLHGHFFQVLSKNGQPISGSPLIKDTLNILPGEEYEIAFKADNTGNWMFHCHDLGHASSGMVTQVNYEGFKPDFTVDPNAGNKPE
Masoomeh Shafiei et al. (2019) β€” Cohnella sp. A01 laccase: thermostable, detergent resistant, anti-environmental and industrial pollutants enzyme
Heliyon  Β· doi:10.1016/j.heliyon.2019.e02543 β†—  Β· Stability - Incubation
16 measurements ▼
Database ID
UniParc: UPI000655361D β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number (residue numbering shifted of 23 compared to article numbering)
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Acetone 25% 100 60 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Acetone 50% 100 46 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Glycerol 25% 100 62 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Glycerol 50% 100 60 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Methanol 25% 100 120 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Methanol 50% 100 113 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Ethanol 25% 100 102 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Ethanol 50% 100 120 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Isopropanol 25% 100 110 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Isopropanol 50% 100 118 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Isobutanol 25% 100 102 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Isobutanol 50% 100 94 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase n-Hexane 25% 100 110 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase n-Hexane 50% 100 190 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Acetic Acid 25% 100 16 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (2.6 dimethoxyphenol radical cation absorbance measurement, 470 nm) in aqueous phase Acetic Acid 50% 100 0 % Incubation: ?, Assay: 10 mM phosphate buffer Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 90Β°C 4 mM 2,6-Dimethoxyphenol 2,6-dimethoxyphenoxy radical β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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