β-xylanase from Thermotoga naphthophila ATCC BAA-489

Enzyme Description

Species

Thermotoga naphthophila ATCC BAA-489 ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

3.2.1.8 ↗

Sequence

Length: 344 amino acids

MKILPSVLILLLGCVPVFSSQNVSLRELAEKLNIYIGFAAVNNFWSLPDAEKYMEIARREFNILTHENQMKWDTIHPERDRYNFTPAEKHVEFAEENGMIVHGHTLVWHNQLPGWLTGREWTREELLNVLEDHIKTVVSHFKGRVKIWDVVNEAVSDSGTYRESVWYKTIGPEYIEKAFRWAKEADPDAILIYNDYSIEEINAKSNFVYNMIKELKEKGVPVDGIGFQMHIDHRGLNYDSFRRNLERFAELGLQIYITEMDVRIPLSGSEEYYLKKQAEVCAKIFEICLKNPAVKAIQFWGFTDKYSWVPGFFKGYGKALLFDENYNPKPCYYAIKEVLEKKRK

Attia Hamid et al. (2019) — Cloning, Purification, and Characterization of Recombinant Thermostable β-Xylanase Tnap_0700 from Thermotoga naphthophila

Applied Biochemistry and Biotechnology · doi:10.1007/s12010-019-03068-0 ↗ · Stability - Incubation

15 measurements ▼

Database ID

UniProt: D2C759 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	10%	100	91	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	20%	100	88	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	30%	100	75	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	10%	100	79	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	20%	100	75	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	30%	100	71	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	10%	100	94	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	20%	100	90	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	30%	100	88	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	10%	100	88	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	20%	100	82	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	30%	100	77	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	10%	100	80	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	20%	100	78	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	30%	100	76	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control

Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	10%	100	91	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	20%	100	88	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	30%	100	75	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	10%	100	79	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	20%	100	75	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	30%	100	71	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	10%	100	94	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	20%	100	90	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	30%	100	88	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	10%	100	88	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	20%	100	82	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	30%	100	77	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	10%	100	80	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	20%	100	78	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	30%	100	76	%	Incubation: ?, Assay: 50 mM citrate-phosphate buffer	Incubation: ?, Assay: 7	Incubation: Room temperature, Assay: 80°C	1% Xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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