GH10 endo-1,4-β-xylanase (gene TnXynB) from Thermotoga naphthophila RKU-10T

Enzyme Description

Species

Thermotoga naphthophila RKU-10T ↗

Extremophile

Yes "hyperthermophilic" organism

EC Number

3.2.1.8 ↗

Sequence

Length: 344 amino acids

MKILPSVLILLLGCVPVFSSQNVSLRELAEKLNIYIGFAAVNNFWSLPDAEKYMEIARREFNILTHENQMKWDTIHPERDRYNFTPAEKHVEFAEENGMIVHGHTLVWHNQLPGWLTGREWTREELLNVLEDHIKTVVSHFKGRVKIWDVVNEAVSDSGTYRESVWYKTIGPEYIEKAFRWAKEADPDAILIYNDYSIEEINAKSNFVYNMIKELKEKGVPVDGIGFQMHIDHRGLNYDSFRRNLERFAELGLQIYITEMDVRIPLSGSEEYYLKKQAEVCAKIFEICLKNPAVKAIQFWGFTDKYSWVPGFFKGYGKALLFDENYNPKPCYYAIKEVLEKKRK

Ikram ul Haq et al. (2019) — Insight into kinetics and thermodynamics of a novel hyperstable GH family 10 endo-1,4-β-xylanase (TnXynB) with broad substrates specificity cloned from Thermotoga naphthophilaRKU-10T

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2019.04.009 ↗ · Stability - Incubation

11 measurements ▼

Database ID

UniProt: D2C759 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (11 measurements)

11 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	100.0	52.3	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetonitrile	50%	100.0	54.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethyl Acetate	50%	100.0	56.2	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Glycerol	50%	100.0	45.41	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	50.3	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	44.7	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Chloroform	50%	100.0	59.12	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	50%	100.0	49.3	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	50%	100.0	46.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	50%	100.0	44.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	50%	100.0	42.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	100.0	52.3	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetonitrile	50%	100.0	54.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethyl Acetate	50%	100.0	56.2	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Glycerol	50%	100.0	45.41	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	50.3	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethylformamide (DMF)	50%	100.0	44.7	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Chloroform	50%	100.0	59.12	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	50%	100.0	49.3	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	50%	100.0	46.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	1-Butanol	50%	100.0	44.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent
Stability - Incubation	Activity after incubation (1 hour at 70°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	50%	100.0	42.8	%	Incubation: ?, Assay: 50% McIlvaine buffer	Incubation: ?, Assay: 6	Incubation: 70°C, Assay: 95°C	2% w/v Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay was really in aqueous conditions or in the presence of organic solvent

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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