Esterase (gene EstGtA3) from Geobacillus thermodenitrificans NG80‐2

Enzyme Description

Species

Geobacillus thermodenitrificans NG80‐2 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.1.1.1 ↗

Sequence

Length: 244 amino acids

MVMAIAAGIMRDYALYSGELQEKIELLVYLPSNFSPLHKYSLLIAQDGKDYFMYGKVKRVIETLMEEGTIDRTIVVGIPYRNVNDRYEKYHPAGQKHEAYIRFLAHELVPFLDHELPTYQMGKGRALIGDSLGGTISLLAGLLYPHTFGNIAMQSPYIDDSVLARIRAFRDPSLLSIYHSVGTEETAVKTTDGHVRDFITPNRVARDLFMEKQFAYTYHEFAGGHAWTYWQPDLPRAVSAILSL

Nicola Curci et al. (2019) — Identification of a novel esterase from the thermophilic bacterium Geobacillus thermodenitrificans NG80-2

Extremophiles · doi:10.1007/s00792-019-01093-9 ↗ · Stability - Incubation

20 measurements ▼

Database ID

UniProt: A4ILC0 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Chloroform	10%	100	51	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100	60	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	49	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100	42	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100	41	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	30%	100	42	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	10%	100	37	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100	53	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	30	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Chloroform	30%	100	34	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	92	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	62	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	93	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100	62	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100	89	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	30%	100	143	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	10%	100	113	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100	40	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	85	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100	50	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase

Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Chloroform	10%	100	51	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	30%	100	60	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethylformamide (DMF)	10%	100	49	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	30%	100	42	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetonitrile	10%	100	41	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	30%	100	42	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	1-Butanol	10%	100	37	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	30%	100	53	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Isopropanol	10%	100	30	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Chloroform	30%	100	34	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	10%	100	92	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	62	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	10%	100	93	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	30%	100	62	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Acetone	10%	100	89	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	30%	100	143	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	n-Hexane	10%	100	113	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	30%	100	40	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Ethanol	10%	100	85	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase
Stability - Incubation	Activity after incubation (1 hour at 55°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase	Methanol	30%	100	50	%	Incubation: ?, Assay: 50 mM Tris–HCl, 4% propan-2-ol	Incubation: ?, Assay: 7.5	Incubation: 55°C, Assay: 55°C	2 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Water-incubated control (in %), assay in aqueous phase

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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