Ξ±-amylase (gene amySL3) from Alkalibacterium sp. SL3.
Enzyme Description
Species
Extremophile
Yes
"extremely halophilic" organism
EC Number
Sequence
MNGTMMQYFEWDLPNDGKHWKRLKEDAEHLNEIGVTAVWIPPCFKSVGQDDVGYGIYDLYDLGEFDQKGTVRTKYGTKDELLEAIEALHEQGIHVYADVVLNHKAGADGTERFEATPVDPENRHEAIGDTREIESWTFFNFPGREGKHSDFEWHWYHFSGVSEDTISEEQGIYRIEGEDKGWAPDDNVSTEFGNFDYLMFADIDYSHPEVVEETKKWIHWFIEETNIDGIRLDAVKHIDSQFMRELIEDVREVHGEEFFFVAEYWHQRYKYLEEYLEEHDYSISMMDVRFHYALHEASKQHNEFDLRRLLDGTLYKTNAEQAVTFVENHDSQPGQSLESYVEPWFKPLAYGFILLSYYGYPCVFYSDYYGYKGDVDYDGCRREIEMLLKARKDYAYGEQYNYLDHGNCIGFTRTGNDEHPEGCAVIMSNGDEGFKEMSVGELHANEVYVDLMNNRDEEIEIDGEGKATFPVNAESISVWVRKTE
Guozeng Wang et al. (2019)
β A New Extremely Halophilic, Calcium-Independent and Surfactant-Resistant Alpha-Amylase from Alkalibacterium sp. SL3
Journal of Microbiology and Biotechnology
Β· doi:10.4014/jmb.1901.01038 β
Β· Activity - Classical
▼
Database ID
UniParc: UPI0010BC24D8 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (18 measurements)
18 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isoamyl Alcohol | 50% | 100.0 | 38.0 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Chloroform | 50% | 100.0 | 83.2 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Chloroform | 20% | 100.0 | 85.1 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | n-Hexane | 50% | 100.0 | 95.9 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | n-Hexane | 20% | 100.0 | 90.0 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Glycerol | 50% | 100.0 | 73.8 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Glycerol | 20% | 100.0 | 85.8 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Acetone | 50% | 100.0 | 0.0 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Acetone | 20% | 100.0 | 6.6 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 0.0 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isoamyl Alcohol | 20% | 100.0 | 89.8 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isobutanol | 50% | 100.0 | 9.5 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Isobutanol | 20% | 100.0 | 90.7 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | 1-Butanol | 50% | 100.0 | 5.4 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | 1-Butanol | 20% | 100.0 | 88.6 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Ethanol | 50% | 100.0 | 3.8 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 3.3 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (DNS method, 540 nm) in the presence of organic solvent | Methanol | 50% | 100.0 | 0.0 | % | 90% McIlvaine buffer (200 mM Na2HPO4 , 100 mM citric acid), 4M NaCl | 7.5 | 45Β°C | 1% Starch | Reducing sugars | β | β | Correct contol (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.