Lipase from Pseudomonas sp. LSK25

Enzyme Description

Species
Pseudomonas sp. LSK25 β†—
Extremophile
Yes "psychrophilic" organism
EC Number
3.1.1.3 β†—

Sequence

Length: 476 amino acids
MGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGSTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQVEVLGKYDGDGKLLEIGIGFRGTSGPRETLISDSIGDLVSDLLAALGPKDYAKNYAGEAFGTLLKDVAAYADSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWAGFYNDSNYVAYASPTQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGAHDKPHESTTDNIVSFNDHYASTLWNVLPFSIVNVPTWLSHLPTGYGDGLTRVLESKFYDLTSRDSTIIVANLSDPARANTWVQNLNRNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIGYQSTDKLVFRDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS
Leelatulasi Salwoom et al. (2019) β€” New Recombinant Cold-Adapted and Organic Solvent Tolerant Lipase from Psychrophilic Pseudomonas sp. LSK25, Isolated from Signy Island Antarctica
International Journal of Molecular Sciences  Β· doi:10.3390/ijms20061264 β†—  Β· Stability - Incubation
11 measurements ▼
Database ID
UniParc: UPI000F706473 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100 101 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Methanol 25% 100 101 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Acetonitrile 25% 100 0 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Ethanol 25% 100 0 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Isopropanol 25% 100 99 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Benzene 25% 100 33 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Toluene 25% 100 232 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase Xylene 25% 100 176 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase n-Hexane 25% 100 226 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase n-Heptane 25% 100 302 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C) measured by absorbance spectrophotometry (colorimetric assay, absorbance measurement of the free fatty acid reacting with cupric acetate–pyridine reagent product, 715 nm) in aqueous phase n-Hexadecane 25% 100 238 % Incubation: ?, Assay: 35 mM potassium phosphate buffer, 0.09 mM CaCl2 Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 30% Olive oil free fatty acids , glycerol β€” Incubation: 200 rpm Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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