Carboxylesterase (gene Est5250) from Bacillus thermocloaceae
Enzyme Description
Species
Extremophile
Yes
"thermoalkaliphilic" organism
EC Number
Sequence
MKHFYRRGKRDGSPVLLLLHGTGGNERDLLPLANILDPEAGVLGVRGEVSENGMARFFRRIREGVFDVEDLVFRTRRLAEFLDSAAEQYAFDRKRVIAVGYSNGANMAASLLFHIPGSLKGALLFHPMVPRRGIALPDLSGTKVWIAAGEEDPICPKEEAEELENLLRDAGSDVTVHWEKGGHSIKKSEVEQARQWYQKHF
Youri Yang et al. (2019)
β Characterization of a novel thermostable carboxylesterase from thermoalkaliphilic bacterium Bacillus thermocloaceae
Bioscience, Biotechnology, and Biochemistry
Β· doi:10.1080/09168451.2019.1574555 β
Β· Activity - Classical
▼
Database ID
UniProt: A0A3G2T988 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetone | 1% | 100.0 | 86.1 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 1% | 100.0 | 93.9 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 1% | 100.0 | 78.4 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1% | 100.0 | 90.9 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 1% | 100.0 | 97.9 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 1% | 100.0 | 80.9 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 1% | 100.0 | 103.1 | % | 50 mM Tris-HCl buffer | 8 | 60Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in %) | Assumprion that assay conditions are the optimal ones (for buffer, pH and temperature |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.