Lipase (gene LIPY8) from Yarrowia lipolytica

Enzyme Description

Species

Yarrowia lipolytica ↗

Extremophile

No but "cold-active" enzyme

EC Number

3.1.1.3 ↗

Sequence

Length: 371 amino acids

MVSLSARIKDFFSVLLLGAATITPSTQTAGVSQGFYDFARDFAHLSNIAYCVNAPITPLNPDFTCGNSCKHFPDIELVKTFGGNFFKTSITGYLAVDHVKKEKYVVFRGTFSLADAITDMQFLLSPFLVDVPALNTFSANDTTAEAQTHCEGCKIHDGFSKAFTETWGNIGEDLQKHLDANPDYQLYVTGHSLGAAVALLGATSIKLKGYDPILINYGQPRVGNKPFAEFINKLWFGEGNGLEITPERKLYRMTHWNDIFVGLPNWEGYTHSNGEVYINNRFINPPLKDVISCAGGENSKCYRSSFSLLSQINLLQNHLAYIDYIGYCALNIGRRELADQEHYTGPYYYGHRSEEDFKKLGLELSTPQVEN

Tang Li et al. (2019) — Cold-active extracellular lipase: Expression in Sf9 insect cells, purification, and catalysis

Biotechnology Reports · doi:10.1016/j.btre.2018.e00295 ↗ · Activity - Classical Stability - Incubation

15 measurements ▼

Database ID

UniProt: Q3HRV6 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host insect Spodoptera frugiperda Sf9 cells

Experimental Data (15 measurements)

15 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	10%	100.0	174.4	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	20%	100.0	5.2	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	100.0	180.3	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100.0	266.7	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100.0	50.1	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100.0	361.5	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	20%	100.0	38.7	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	416.9	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	—	Correct contol (in %)
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100.0	72.81	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	20%	100.0	0.9	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	20%	100.0	1.38	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	20%	100.0	0.0	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	88.05	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethylformamide (DMF)	20%	100.0	13.21	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Diethyl Ether	20%	100.0	10.86	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid , p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	10%	100.0	174.4	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Isopropanol	20%	100.0	5.2	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	100.0	180.3	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100.0	266.7	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100.0	50.1	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	10%	100.0	361.5	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Acetone	20%	100.0	38.7	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	416.9	%	50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	7.5	22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	—	Correct contol (in %)
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	20%	100.0	72.81	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	20%	100.0	0.9	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetone	20%	100.0	1.38	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	20%	100.0	0.0	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	20%	100.0	88.05	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethylformamide (DMF)	20%	100.0	13.21	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent
Stability - Incubation	Activity after incubation (2 hours at 22°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Diethyl Ether	20%	100.0	10.86	%	Incubation: ?, Assay: 50 mM sodium phosphate buffer, 0.2% (w/v) Nadeoxycholate, 0.1% (w/v) gum arabic	Incubation: ?, Assay: 7.5	Incubation: 22°C, Assay: 22°C	0.03% (w/v) p-Nitrophenyl laurate	Lauric Acid, p-Nitrophenol	—	Incubation: 200 rpm	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay in aqueous phase or in the presence of organic solvent

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation