Laccase (gene Lac1326) from a marine sediment metagenomic library

Enzyme Description

Species
Na (marine sediment metagenomic library)
Extremophile
No likely not for organism, but "thermostable" and "salt-tolerant" enzyme
EC Number
1.10.3.2 β†—

Sequence

Length: 441 amino acids
MLAFDFFLQASAGSILPLQALAKADPLEVAEPVTAQILPEGEGTTAMLGFMGSTPGPELRARQGEQFDIDVQNRIEEGSAVHWHGARRDDNMDGVPVMTQDLIEPGDEKEYSFAAPDAATIWYHSHQGSAEQVARGTRGPLIVEMGPPDVDHGDLTVLPDWWIIHEDGVLSDEFTNMSGDAHRGYLGNFARASLSPVEEVAGDDRVRSRVINAATDRIFPLGRQGVDGTVVGLDGMTLVSPRQPSDLTLAPAQRADHIMDATSPDPVGFGLISRDGRYFLPAHDVFGSNTARWPFWIVPLLPNLADEPDAKGHVTLTMGTEDTPLHPLPQAGMMSANIWAFNFLEILPDAPFHSPKRGETHRIQLYNDTGFPHGIHLHGHHFAADGADGSLGFLRDTTLVPAGEQRDLNCVFDNPGRWLVHCHMLGMALGGMDTWVPDAPL
Qihao Yang et al. (2018) β€” Characterization of a Novel, Cold-Adapted, and Thermostable Laccase-Like Enzyme With High Tolerance for Organic Solvents and Salt and Potent Dye Decolorization Ability, Derived From a Marine Metagenomic Library
Frontiers in Microbiology  Β· doi:10.3389/fmicb.2018.02998 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
UniParc: UPI00065B269B β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 10% 100.0 158.5 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 30% 100.0 115.2 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 50% 100.0 109.6 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 10% 100.0 237.2 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 30% 100.0 189.2 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 50% 100.0 135.6 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 10% 100.0 170.9 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 30% 100.0 106.4 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 50% 100.0 89.2 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 10% 100.0 103.7 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 30% 100.0 82.2 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 50% 100.0 39.7 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100.0 116.4 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100.0 87.1 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 25Β°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 73.8 % Incubation: ?, Assay: 40 mM Britton-Robinson buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 65Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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