Laccase-like multicopper oxidase (gene lmco1) from Thermothelomyces thermophila

Enzyme Description

Species

Thermothelomyces thermophila ↗

Extremophile

Yes "thermophilic" organism

EC Number

1.10.3.2 ↗ (LMCO activity)

Sequence

Length: 650 amino acids

MLLSKLSILLAKWLSVAVYAGTLVHDEQFIPDHILRVSVAQVPSACENREDVVVNGTSPGPAIHLLPGARTWIRVYNDMNDRNLSMHWHGLSQRFAPFSDGTPSATQWPIPPGHFFDYEILTEPEDAGTYFYHSHVGMQALSCTGPLIVEDCGSSPYHYDDERILLFQDHFQKSDLEMIQGLTSTQFTWTGETRGILLNGRGVSPNQAAVQGRPGEASGFFGSHRFSNFRAGDGTSNSWDGIRGDDQIEPPTDCTLPVIDVEPGKTYRLRFIGATGLSLLTMGFEDHNDLTIVQVDGSEYNAPVTVDHIQLGGGQRFDVLLRTKTAEELRCNGDKTTYFLQFETRDRPDPYRGYGVLRYNLGTPVPAAPTTPALTLPAEVNNWLEYTFQPLHPSSSLSPTAEEVTRRVILEAEQKIDPATGRLVWKLAHMTWTDMSRDKPVLVDIYERGEAAMPDYAAALTNYGWDPATKLFPAKKDEVLEIVIQNTGSHYSGASGIVETHPFHAHGQHFYDVGSGPGKYDPEANNAKLASLGYRPIKRDTTMVYRYGEGKVAPGEPAGWRAWRMKMNNPGVWMVHCHILAHMIMGMETIWVVGDAEDIVTIPLSVSQNYFTYGGSVYGNDTHAPEVYHYFDDTNKCCAAGAGDSEDSGH

Anastasia Zerva et al. (2019) — A novel thermophilic laccase-like multicopper oxidase from Thermothelomyces thermophila and its application in the oxidative cyclization of 2',3,4-trihydroxychalcone

New Biotechnology · doi:10.1016/j.nbt.2018.12.001 ↗ · Activity - Classical

6 measurements ▼

Database ID

UniProt: G2QFD0 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Pichia pastoris X33

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Ethanol	10%	100.0	76.0	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Ethanol	50%	100.0	27.0	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Methanol	10%	100.0	79.8	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Methanol	50%	100.0	44.4	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	51.9	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Acetone	10%	100.0	51.5	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Ethanol	10%	100.0	76.0	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Ethanol	50%	100.0	27.0	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Methanol	10%	100.0	79.8	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Methanol	50%	100.0	44.4	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	51.9	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 469 nm) in the presence of organic solvent	Acetone	10%	100.0	51.5	%	50 mM phosphate – citrate buffer	4	40°C	2 mM ABTS	ABTS radical cation	—	—	Correct contol (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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