Polyethylene terephthalate hydrolase (gene IsPETase) from Ideonella sakaiensis
Enzyme Description
Sequence
MNFPRASRLMQAAVLGGLMAVSAAATAQTNPYARGPNPTAASLEASAGPFTVRSFTVSRPSGYGAGTVYYPTNAGGTVGAIAIVPGYTARQSSIKWWGPRLASHGFVVITIDTNSTLDQPSSRSSQQMAALRQVASLNGTSSSPIYGKVDTARMGVMGWSMGGGGSLISAANNPSLKAAAPQAPWDSSTNFSSVTVPTLIFACENDSIAPVNSSALPIYDSMSRNAKQFLEINGGSHSCANSGNSNQALIGKKGVAWMKRFMDNDTRYSTFACENPNSTRVSDFRTANCS
Congcong Liu et al. (2019)
β Structural and functional characterization of polyethylene terephthalate hydrolase from Ideonella sakaiensis
Biochemical and Biophysical Research Communications
Β· doi:10.1016/j.bbrc.2018.11.148 β
Β· Activity - Classical
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Database ID
UniProt: A0A0K8P6T7 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (4 measurements)
4 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 10% | 1.4 | 1.0 | mM/min | ? | ? | 30Β°C | ? mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in mM/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Ethanol | 30% | 1.4 | 0.09 | mM/min | ? | ? | 30Β°C | ? mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in mM/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Isopropanol | 10% | 1.4 | 1.1 | mM/min | ? | ? | 30Β°C | ? mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in mM/min) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement) in the presence of organic solvent | Isopropanol | 30% | 1.4 | 0.15 | mM/min | ? | ? | 30Β°C | ? mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Correct contol (in mM/min) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.