Lipase (gene lipPN1) from Proteus sp. NH 2-2
Enzyme Description
Sequence
MSTTYPIVLVHGLSGFGDIVGYPYFYGIADALEKDGHKVFTASLSAFNSNEVRGEQLWEFVQKVLKETKAKEVNLIGHSQGPLACRYVAAKHAKSIASVTSVNGVNHGSEIADLVRRIMRKDSVPEYIADAVMKAIGTIISTFSGNRGNPQDAVAALEALTTENVTEFNKKYPQGLPAVRGGEGKEIVNGVHYYSFGSYIQGLIAGEKGNLLDPTHAAMRILSAFFSERENDGLVGRTSMRLGKLIKDDYAEDHLDMVNQVAGLVGRGEDIIAIYTNHANFLASKKL
Hua Shao et al. (2018)
β Gene cloning, expression in E. coli, and in vitro refolding of a lipase from Proteus sp. NH 2-2 and its application for biodiesel production
Biotechnology Letters
Β· doi:10.1007/s10529-018-2625-1 β
Β· Stability - Incubation
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Database ID
UniParc: UPI000D777649 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (10 measurements)
10 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 10% | 100.0 | 82.31 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 30% | 100.0 | 48.53 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 10% | 100.0 | 104.6 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 30% | 100.0 | 16.18 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 10% | 100.0 | 178.79 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Acetone | 30% | 100.0 | 70.67 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 10% | 100.0 | 47.38 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 30% | 100.0 | 26.38 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 10% | 100.0 | 72.25 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 30% | 100.0 | 11.43 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 9, Assay: 8 | Incubation: Room temperature, Assay: 65Β°C | 1 mM p-Nitrophenyl butyrate | Butyric Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.