Choline oxidase from Arthrobacter globiformis
Enzyme Description
Sequence
MHIDNIENLSDREFDYIVVGGGSAGAAVAARLSEDPAVSVALVEAGPDDRGVPEVLQLDRWMELLESGYDWDYPIEPQENGNSFMRHARAKVMGGCSSHNSCIAFWAPREDLDEWEAKYGATGWNAEAAWPLYKRLETNEDAGPDAPHHGDSGPVHLMNVPPKDPTGVALLDACEQAGIPRAKFNTGTTVVNGANFFQINRRADGTRSSSSVSYIHPIVEQENFTLLTGLRARQLVFDADRRCTGVDIVDSAFGHTHRLTARNEVVLSTGAIDTPKLLMLSGIGPAAHLAEHGIEVLVDSPGVGEHLQDHPEGVVQFEAKQPMVAESTQWWEIGIFTPTEDGLDRPDLMMHYGSVPFDMNTLRHGYPTTENGFSLTPNVTHARSRGTVRLRSRDFRDKPMVDPRYFTDPEGHDMRVMVAGIRKAREIAAQPAMAEWTGRELSPGVEAQTDEELQDYIRKTHNTVYHPVGTVRMGAVEDEMSPLDPELRVKGVTGLRVADASVMPEHVTVNPNITVMMIGERCADLIRSARAGETTTADAELSAALA
Rachel S. Heath et al. (2018)
β An Engineered Alcohol Oxidase for the Oxidation of Primary Alcohols
ChemBioChem
Β· doi:10.1002/cbic.201800556 β
Β· Activity + Stability - Conversion
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Database ID
UniProt: Q7X2H8 β
Sequence Annotation
Explicit - Provided PDB Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (12 measurements)
12 measurements
| Mutation | Property | Assay | Solvent | Solvent Volume | Aqueous Reference | WT Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Ethyl Acetate | 50% | 22 | β | 2 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| WT | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | n-Heptane | 50% | 22 | β | 19 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| WT | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Cyclohexane | 50% | 22 | β | 4 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| WT | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | tert-Butyl Methyl Ether (MTBE) | 50% | 22 | β | 2 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| WT | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Toluene | 50% | 22 | β | 0 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| WT | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Dichloromethane | 50% | 22 | β | 0 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| F357R/M359R | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Ethyl Acetate | 50% | 54 | 2 | 70 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| F357R/M359R | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | n-Heptane | 50% | 54 | 19 | 87 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| F357R/M359R | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Cyclohexane | 50% | 54 | 4 | 83 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| F357R/M359R | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | tert-Butyl Methyl Ether (MTBE) | 50% | 54 | 2 | 34 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| F357R/M359R | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Toluene | 50% | 54 | 0 | 32 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
| F357R/M359R | Activity + Stability - Conversion | Conversion after incubation (24 hours at 30Β°C) in the presence of organic solvent, measured by gas chromatography | Dichloromethane | 50% | 54 | 0 | 14 | % | 100 mm potassium phosphate buffer | 8 | 30Β°C | 1-Hexanol, Molecular oxygen | HβOβ (Hydrogen Peroxide) , Hexanal | FAD | 200 rpm | Classical aqueous control (in %) |
Mutations in this dataset (2)
WT
F357R/M359R
Visualization : Activity + Stability β Conversion
One bar per measurement. Colour = solvent, shade = solvent volume. β β β Reference value. Hover for details.
Mutation Effect
Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.