Esterase (gene BlEst1) from Bacillus licheniformis ATCC 14580
Enzyme Description
Extremophile
No
but "halotolerant" enzyme
EC Number
Sequence
MKIVKPQPFTFKGGKKAVLLLHGFTGNTADVRMLGRYLNEKGYTCHAPQYKGHGVPPEELLSTGPEDWWKDVMDGYEYLKSEGYEQIAACGLSLGGVFSLKLGYTVPIKGIVPMCAPMYIKSEETMYEGVLDYARNYKKFEGKTAEQINAEMEEFKKTPMNTLKALQDLIADVREHVDMIYSPTFVVQARHDHMINTDSANIIYNEVETDDKQLKWYEESGHAITLDKERETLHKDVYQFLETLDWQT
Aline M. Nakamura et al. (2018)
β Low-resolution envelope, biophysical analysis and biochemical characterization of a short-chain specific and halotolerant carboxylesterase from Bacillus licheniformis
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2018.10.003 β
Β· Activity - Classical
▼
Database ID
UniProt: Q65EQ1 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli Rosetta (DE3)
Experimental Data (5 measurements)
5 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 1% | 100.0 | 97.3 | % | 30 mM Tris-HCl buffer, 150 mM NaCl | 7 | 40Β°C | 1 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 1% | 100.0 | 89.32 | % | 30 mM Tris-HCl buffer, 150 mM NaCl | 7 | 40Β°C | 1 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | 1-Butanol | 1% | 100.0 | 104.67 | % | 30 mM Tris-HCl buffer, 150 mM NaCl | 7 | 40Β°C | 1 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Glycerol | 1% | 100.0 | 88.77 | % | 30 mM Tris-HCl buffer, 150 mM NaCl | 7 | 40Β°C | 1 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 1% | 100.0 | 85.14 | % | 30 mM Tris-HCl buffer, 150 mM NaCl | 7 | 40Β°C | 1 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.