Esterase (gene ML-005) from an oil-contaminated soil sample metaproteomic library

Enzyme Description

Species

Na (oil-contaminated soil sample metaproteomic library)

Extremophile

No but "halotolerant" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 215 amino acids

MIRKLILLVLFGFMSFVANAKTIELRENNMRQIFVLHGYSASIDDHWFLDLKHQIEDEHTTVTLIPFPDSEHPDVDAWQKVLDQHIPVVNENTYFVAHSLGVITLLHFLQRHDYQKIGGMILVSGFSGPISDFSPLDAYITKSKVDTSYFHDIKKKLVYLSDNDDLVPPKLTIQLAKEIDAPYITVPNGGHFLGREGYTTFPQVVNSLKEMLESK

Premankur Sukul et al. (2018) — Characterization of ML-005, a Novel Metaproteomics-Derived Esterase

Frontiers in Microbiology · doi:10.3389/fmicb.2018.01925 ↗ · Activity - Classical

10 measurements ▼

Database ID

ENA: ERP017906 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	1%	100	67	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	21	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	1%	100	51	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100	8	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	53	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	10	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	100	30	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	1	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	1%	100	24	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100	1	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	1%	100	67	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	21	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	1%	100	51	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100	8	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	53	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	10	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	100	30	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	1	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	1%	100	24	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100	1	%	50 mM sodium phosphate buffer	8	25°C	50 µM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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