Esterase (gene EstSP2) from Sphingomonas glacialis PAMC 26605

Enzyme Description

Species
Sphingomonas glacialis PAMC 26605 β†—
Extremophile
Yes "psychrophilic" organism
EC Number
3.1.1.1 β†—

Sequence

Length: 315 amino acids
MTDLSLVDPELRPMLDQWPTLALDADLLATIRAAGSRLPAPPVVPNAVTCERRSVPGPAGAPDVSLLIYTPAGTGPFPCLFHIHGGGYVMGDAASLEFAHRPMAEALGCVIVSVDYRLAPETVFPGAIEDCYAGLAWTFAQATELRVDPARIGVIGESAGGGLAAALALLARDRGTYRLAFQHLTYPMLDDRTCTRDDPNPATGQFIWTPHNNRFGWRALLGRDPGADDVSPYAAAARATDLAGLPPTYIATGALDLFVDEDLDYARRLIRAGVPTELHVYPGGFHGFDIDPNAAVAAQARRDRMDALRRAMVVK
Vinay Kumar Dachuri et al. (2018) β€” Organic Solvent-Tolerant Esterase from Sphingomonas glacialis Based on Amino Acid Composition Analysis: Cloning and Characterization of EstSP2
Journal of Microbiology and Biotechnology  Β· doi:10.4014/jmb.1806.06032 β†—  Β· Activity + Stability - Incubation
20 measurements ▼
Database ID
UniParc: UPI00026CCA71 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 30% 100 101 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 40% 100 7 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 30% 100 27 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 20% 100 48 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Isopropanol 10% 100 72 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 40% 100 7 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 30% 100 9 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 20% 100 29 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetone 10% 100 48 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 40% 100 85 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100 103 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 20% 100 99 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 10% 100 100 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 40% 100 62 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 30% 100 93 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 20% 100 100 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10% 100 93 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40% 100 77 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100 78 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature
Activity + Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100 102 % ? Incubation, 100 mM Tris-HCl, 100 mM NaCl, 5% glycerol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 40Β°C ? mM p-Nitrophenol acetate Acetic Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in the presence of organic solvent | Clear assay in organic solvent, uncertainty about temperature

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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