GH1 β-glucosidase (gene bgl) from Alteromonas sp. L82

Enzyme Description

Species

Alteromonas sp. L82 ↗

Extremophile

No but"cold-adapted" enzyme

EC Number

3.2.1.21 ↗

Sequence

Length: 446 amino acids

MTKKLSLPSTSPMMQPSFLFGTATSSFQIEGDAEGRLECIWDVFCRQAGAIADKSDGLRACEHVERWEEDVNLIHSLGVDAYRLSISWPRVMHANGEINEQGLAFYKQLIDKLNSLNIKAFVTLYHWDLPQYLEQRGGWLNRQTAYEFENYVDKISAALGERVYSYATFNEPFCSAYLGYEIGVHAPGKTGREHGRTAAHHILLAHGLGMKMLKKNVPNAQNGIVLNFTPCYSASDSPADIAATKKADQYINQWYMQPVMEGCYPDVIHELTEKDKPPIQDGDMETICQPLDFLGINFYTRLHYSAPRNHDELYFEHPHYEPKTDIGWEIHPPALTHLLTSLHQRYALPPVYITENGAAMADEVVDGRVEDNDRIEYYQNHLLAVEKAMDEGVKVSGYFAWSLMDNFEWAEGYEKRFGIVYVDFDTQSRILKDSAKAFKALLAQRT

Jingjing Sun et al. (2018) — Overexpression and characterization of a novel cold-adapted and salt-tolerant GH1 β-glucosidase from the marine bacterium Alteromonas sp. L82

Journal of Microbiology · doi:10.1007/s12275-018-8018-2 ↗ · Activity - Classical

6 measurements ▼

Database ID

UniParc: UPI000E8F2200 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	67.6	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100.0	2.1	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	2.0	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	57.9	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	5.7	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	1.7	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	67.6	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100.0	2.1	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100.0	2.0	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	57.9	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	5.7	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100.0	1.7	%	50 mM sodium phosphate buffer	7	40°C	5 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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