Azoreductase (gene azoA) from Bacillus sp. AO1
Enzyme Description
Species
Extremophile
Yes
"alkaliphilic" organism
EC Number
1.7.1.6 β (author annotation in conflict with UniProt)
Sequence
MTKVLYITAHPHDDTQSFSMAVGKAFIDTYKEVNPDHEVETIDLYIEDIPHIDVDVFSGWGKLRSGQGFDQLSSDEKAKVGRLSELCEQFVSADKYIFVSPLWNFSFPPVLKAYIDSVAVAGKTFKYTEQGPVGLLTDKKALHIQARGGIYSEGPAAQMEMGHRYLSIIMQFFGVPSFDGLFVEGHNAMPDKAQEIKEKAVARAKDLAHTF
Hirokazu Suzuki et al. (2018)
β Azoreductase from alkaliphilic Bacillus sp. AO1 catalyzes indigo reduction
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-018-9284-y β
Β· Activity - Classical
▼
Database ID
UniProt: A0A455X8R2 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (6 measurements)
6 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement) | Ethanol | 10% | 100 | 112 | % | 100 mM Tris-HCl buffer | 7.5 | 30Β°C | 1 mM Indigo carmine | Reduced indigo | 2 mM NADH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement) | 1-Propanol | 10% | 100 | 160 | % | 100 mM Tris-HCl buffer | 7.5 | 30Β°C | 1 mM Indigo carmine | Reduced indigo | 2 mM NADH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement) | Isopropanol | 10% | 100 | 141 | % | 100 mM Tris-HCl buffer | 7.5 | 30Β°C | 1 mM Indigo carmine | Reduced indigo | 2 mM NADH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement) | Propylene Glycol | 10% | 100 | 155 | % | 100 mM Tris-HCl buffer | 7.5 | 30Β°C | 1 mM Indigo carmine | Reduced indigo | 2 mM NADH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement) | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 211 | % | 100 mM Tris-HCl buffer | 7.5 | 30Β°C | 1 mM Indigo carmine | Reduced indigo | 2 mM NADH | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (NADPH absorbance measurement) | Dimethylformamide (DMF) | 10% | 100 | 243 | % | 100 mM Tris-HCl buffer | 7.5 | 30Β°C | 1 mM Indigo carmine | Reduced indigo | 2 mM NADH | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.