Endo-1,4-Ξ²-mannanase (gene ManEM17) from a Hermetia illucens gut metagenomic library
Enzyme Description
Species
Na (Hermetia illucens gut metagenomic library)
Extremophile
No
but "inhibitor-resistant" enzyme
EC Number
Sequence
MKKNLFSTKLLLIISFALLVFSCKDDDEVTGTQRYKEPPVLLSSIPATGGTVSFADNSIRLIFDREVTVVDLGMVSVNNAIISGSSISGDTLIFTLDGIDEKTQYTLTIAKGAIKGVPGLLNQEDITVSFNTGEAPALTKTLVTPNASAQAKNVFNFLLENYRNKIISGSMARVNWNTDEADRVYRWTGKYPAINTFDFVHLYASPASWIDYSNTTVAENWWNNKGLVSIMWHWNVPIEGTSNFGFYYTGKNNGEGETSFDITKAVQDGTDENKIIKADLDKVATHLLALQAKGIPVIWRPLHEAAGGWFWWGAKDANSYKALWILMFDTFKAKGINNLIWVWTTETNDNSWYPGDAYVDIIGRDIYNKKDAAAIYKEYLSIRKTYPGKMVTLSECGNVANISQQWSANAKWSWYMPWYDYNATDESEHSHATKAFWTDAFASDKVITRDQMPSLK
Jaeeun Song et al. (2018)
β Characterization of an inhibitor-resistant endo-1,4-Ξ²-mannanase from the gut microflora metagenome of Hermetia illucens
Biotechnology Letters
Β· doi:10.1007/s10529-018-2596-2 β
Β· Stability - Incubation
▼
Database ID
UniProt: A0A1W6DZL1 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (16 measurements)
16 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 100 | 101 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Dimethylformamide (DMF) | 10% | 100 | 94 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 10% | 100 | 95 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetonitrile | 10% | 100 | 56 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 10% | 100 | 94 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Acetone | 10% | 100 | 93 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Isopropanol | 10% | 100 | 94 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Butanol | 10% | 100 | 0 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Pentanol | 10% | 100 | 9 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Hexanol | 10% | 100 | 6 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Chloroform | 10% | 100 | 0 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Benzene | 10% | 100 | 0 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | 1-Heptanol | 10% | 100 | 5 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Toluene | 10% | 100 | 5 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Cyclohexane | 10% | 100 | 7 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (30 minutes at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | n-Hexane | 10% | 100 | 4 | % | Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM mM sodium phosphate buffer | Incubation: 6.5, Assay: 6.5 | Incubation: 40Β°C, Assay: 55Β°C | 0.5% (w/v) Locust bean gum | Reducing sugars | β | β | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.