Esterase (gene EstN7) from Bacillus cohnii strain N1
Enzyme Description
Species
Extremophile
No
but"cold-adapted" enzyme
EC Number
Sequence
MKNRIDPELRAMLDMFPPLNLDDVQATRKAMEEAAQLTELPVDEEVVVSNRMVPGPEDNPYVRVRIYEPKEKIEKLPGLLWIHGGGYVLGAPEGDDLLCQRFVKEANCVVVSVDYRLAPEHPYPAPLEDCYAALQWFAKKVDELGVDASRIGVGGQSAGGGLTAALALLARDRKGPELCFQMPLYPMIDDKNNSPSSLEITGNLIWNHDLNEKGWSMYLDGKNGTDDVPVHAAPARATDLTNLPYTYTCVGQLDPFRDETLDYVKRLCQAGVDVEFHLYPGAYHGFETLNPAAAVSQRALAEYVGAVKHVLNREKVVERK
Nehad Noby et al. (2018)
β Cloning, expression and characterization of cold active esterase (EstN7) from Bacillus cohnii strain N1: A novel member of family IV
International Journal of Biological Macromolecules
Β· doi:10.1016/j.ijbiomac.2018.07.169 β
Β· Stability - Incubation
▼
Database ID
UniProt: A0A2K9UV39 β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (8 measurements)
8 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 105.6 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Methanol | 30% | 100.0 | 95.8 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Ethanol | 30% | 100.0 | 99.3 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Isopropanol | 30% | 100.0 | 91.15 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Petroleum Ether | 30% | 100.0 | 129.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Chloroform | 30% | 100.0 | 109.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | Toluene | 30% | 100.0 | 230.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
| Stability - Incubation | Activity after incubation (3 hours at 20Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase | n-Hexane | 30% | 100.0 | 134.0 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 8 | Incubation: 20Β°C, Assay: 20Β°C | 0.5 mM p-Nitrophenol acetate | Acetic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.