Endochitinase (gene mchit1) from Lecanicillium lecanii 43H

Enzyme Description

Species
Lecanicillium lecanii 43H β†—
Extremophile
No
EC Number
3.2.1.14 β†—

Sequence

Length: 383 amino acids
AVYFTNWGIYGRNFQPADLPASEITHVLYSFMNIRSDGTIFSGDTYADYEKHYPGDSWNDVGNNAYGCVKQLYLLKKQNRNLKVMLSIGGWTWSTNFPAAASSATSRKTFAQSAVGFMKDWGFDGIDIDWEYPADATQAQNMVLLLQAVRDELDSYASQYAKGHHFLLSIAAPAGPDNYNKLKLADLGKVLDYVNLMAYDFAGSWSNFTGHDANMYASKDNANATPFNTNDAVQAYIKGGVPASKIVLGMPIYGRSFEKTEGIGKPYNGIGSGSWENGVWDYKALPKSGATVKCDNAVVGCYSYDASTQELISFDTPAVINTKVSWLKGQGLGGSMFWEASADKKGADSLISTSHQGLGSLDSAQNCLDYPNSKYDNIKNGMK
Huu Quan Nguyen et al. (2018) β€” High-level expression, purification and properties of an Endochitinase gene without signal peptide from Lecanicillium lecanii 43H in Pichia pastoris
Molecular Biology Reports  Β· doi:10.1007/s11033-018-4256-y β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
UniProt: A6XBL0 β†—
Sequence Annotation
Explicit - Provided (40 first residues from UniProt sequence absent from the mature protein)
Protein Source
Recombinant, host yeast Pichia pastoris X33

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 10% 100.0 110.0 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 20% 100.0 102.2 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 30% 100.0 50.6 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 10% 100.0 93.4 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 20% 100.0 91.8 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 30% 100.0 25.8 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 10% 100.0 98.9 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 20% 100.0 90.4 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 30% 100.0 33.0 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 10% 100.0 120.8 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 20% 100.0 108.7 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 30% 100.0 29.7 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 10% 100.0 93.7 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 20% 100.0 76.7 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control
Stability - Incubation Activity after incubation (2 hours at 40Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Butanol 30% 100.0 61.7 % Incubation: ?, Assay: 20 mM potassium phosphate buffer Incubation: ?, Assay: 6 Incubation: 40Β°C, Assay: 40Β°C 0.5% (w/v) Colloidal Chitin Reducing sugars β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether the assay was in aqueous phase or in the presence of organic solvent, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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