Phosphotriesterase from Pseudomonas diminuta
Enzyme Description
Sequence
MQTRRVVLKSAAAAGTLLGGLAGCASVAGSIGTGDRINTVRGPITISEAGFTLTHEHICGSSAGFLRAWPEFFGSRKALAEKAVRGLRRARAAGVRTIVDVSTFDIGRDVSLLAEVSRAADVHIVAATGLWFDPPLSMRLRSVEELTQFFLREIQYGIEDTGIRAGIIKVATTGKATPFQELVLKAAARASLATGVPVTTHTAASQRDGEQQAAIFESEGLSPSRVCIGHSDDTDDLSYLTALAARGYLIGLDHIPHSAIGLEDNASASALLGIRSWQTRALLIKALIDQGYMKQILVSNDWLFGFSSYVTNIMDVMDRVNPDGMAFIPLRVIPFLREKGVPQETLAGITVTNPARFLSPTLRAS
Keith E. LeJeune et al. (1997)
β Dramatically stabilized phosphotriesteraseβpolymers for nerve agent degradation
Biotechnology and Bioengineering
Β· doi:10.1002/(SICI)1097-0290(19970420)54:2%3C105::AID-BIT2%3E3.0.CO;2-P β
Β· Activity - Classical Stability - Incubation Stability - Half-life
▼
Database ID
UniProt: P0A434 β
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, bacterium Pseudomonas diminuta
Experimental Data (22 measurements)
22 measurements β page 1 of 2
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 20% | 100.0 | 18.0 | % | 0.12 M Hepes buffer, 50 Β΅litre CoCl2 | 7.4 | 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 30% | 100.0 | 4.0 | % | 0.12 M Hepes buffer, 50 Β΅litre CoCl2 | 7.4 | 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 40% | 100.0 | 2.0 | % | 0.12 M Hepes buffer, 50 Β΅litre CoCl2 | 7.4 | 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 0.0 | % | 0.12 M Hepes buffer, 50 Β΅litre CoCl2 | 7.4 | 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 42.0 | % | 0.12 M Hepes buffer, 50 Β΅litre CoCl2 | 7.4 | 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Stability - Half-life | Enzyme half-life (in days), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) | Dimethyl Sulfoxide (DMSO) | 20% | 1.5 | 1.0 | hours | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 50Β°C, Assay: 25Β°C | ? mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) |
| Stability - Half-life | Enzyme half-life (in days), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) | Dimethyl Sulfoxide (DMSO) | 20% | 1.8 | 7.1 | hours | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | ? mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Classical aqueous control (in hours) |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 10% | 33.0 | 50.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (1 day at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 56.0 | 89.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (2 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 45.0 | 69.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 20% | 33.0 | 64.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (1 day at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 56.0 | 85.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (2 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 45.0 | 61.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 33.0 | 59.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (1 day at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 40% | 56.0 | 96.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (2 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 40% | 45.0 | 71.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 40% | 33.0 | 62.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (1 day at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60% | 56.0 | 39.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (2 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60% | 45.0 | 34.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
| Stability - Incubation | Activity after incubation (3 days at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 412 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 60% | 33.0 | 29.0 | % | Incubation: 0.12 M Hepes buffer, 50 Β΅M CoCl2, Assay: 0.12 M Hepes buffer, 50 Β΅M CoCl2 | Incubation: 7.4, Assay: 7.4 | Incubation: 25Β°C, Assay: 25Β°C | 0.5 mM Paraoxon | diethyl phosphate , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("assayed with 0.5 mM substrate in buffer with less than 1% DMSO") |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Half-life
One bar per measurement. Colour = solvent, shade = solvent volume.