Lipase 2 from Bacillus thermocatenulatus

Enzyme Description

Species
Bacillus thermocatenulatus β†—
Extremophile
Yes thermophilic organism
EC Number
3.1.1.3 β†—

Sequence

Length: 389 amino acids
AASPRANDAPIVLLHGFTGWGREEMLGFKYWGGVRGDIEQWLNDNGYRTYTLAVGPLSSNWDRACEAYAQLVGGTVDYGAAHAAKHGHARFGRTYPGLLPELKRGGRVHIIAHSQGGQTARMLVSLLENGSQEEREYAKAHNVSLSPLFEGGHHFVLSVTTIATPHDGTTLVNMVDFTDRFFDLQKAVLKAAAVASNVPYTSQVYDFKLDQWGLRRQPGESFDHYFERLKRSPVWTSTDTARYDLSIPGAEKLNQWVQASPNTYYLSFSTERTHRGALTGNYYPELGMNAFSAVVCAPFLGSYRNEALGIDDRWLENDGIVNTVSMNGPKRGSSDRIVPYDGTLKKGVWNDMGTCNVDHLEVIGVDPNPSFDIRAFYLRLAEQLASLRP
Seyed Hossein Khaleghinejad et al. (2016) β€” Study the effect of F17S mutation on the chimeric Bacillus thermocatenulatus lipase
Journal of Genetic Engineering and Biotechnology  Β· doi:10.1016/j.jgeb.2016.08.002 β†—  Β· Stability - Incubation
10 measurements ▼
Database ID
UniProt: Q59260 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number (28 first residues from UniProt sequence removed from mature protein)
Protein Source
Recombinant, host bacterium Escherichia coli DH5alpha

Experimental Data (10 measurements)

10 measurements
Mutation Property Assay Solvent Solvent Volume Aqueous ReferenceWT Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase Methanol 30% 77 β€” 97 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
F17S/A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase Methanol 30% 100 β€” 129 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase Acetone 30% 77 β€” 68 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
F17S/A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase Acetone 30% 100 β€” 102 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase n-Heptane 30% 77 β€” 98 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
F17S/A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase n-Heptane 30% 100 β€” 137 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase n-Hexane 30% 77 β€” 114 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
F17S/A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase n-Hexane 30% 100 β€” 83 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase Chloroform 30% 77 β€” 104 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control
F17S/A112G/H113E/Q115A Stability - Incubation Activity after incubation (1 hour at room temperature), measured by pH-STAT (free fatty acids titration) in aqueous phase Chloroform 30% 100 β€” 137 % ? Incubation, ? Buffer, 20 mg/ml arabic gum Incubation: ?, Assay: 9.5 Incubation: Room temperature, Assay: 55Β°C 2 mg/ml Tricaprylin 3-carpylic acid , glycerol β€” β€” Non-incubated control (in %), assay in aqueous phase | Chimeric lipase residual activity defined as 100%/Uncertainty about whether assay in aqueous phase or in the presence of organic solvent, uncertainty about control

Mutations in this dataset (2)

F17S/A112G/H113E/Q115A A112G/H113E/Q115A

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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