Nucleoside 2β€²-deoxyribosyltransferase (gene CtNDT) from Chroococcidiopsis thermalis PCC 7203

Enzyme Description

Species
Chroococcidiopsis thermalis PCC 7203 β†—
Extremophile
Yes "thermoacidophilic" organism
EC Number
2.4.2.6 β†—

Sequence

Length: 155 amino acids
MKRKIIYLASPYGFSQQQKTLLLPPIVRALEALGIEVWEPFARNNQIDFSQADWAYRVAQADLQDVKNCDGIFAVVNGTPPDEGVMVELGMAIALNKAIFLFRDDFRRCSDNERYPLNLMLFAGLPEIGWENYYYTSVDEIQSHDKALYKWLTGM
Jon Del Arco et al. (2018) β€” Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2'-deoxyribosyltransferase from Chroococcidiopsis thermalis
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-018-9134-y β†—  Β· Activity - Classical
12 measurements ▼
Database ID
UniProt: K9TVX3 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Methanol 20% 100 100 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Ethanol 20% 100 100 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Isopropanol 20% 100 100 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Glycerol 20% 100 65 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Ethylene Glycol 20% 100 95 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Propylene Glycol 20% 100 90 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Acetonitrile 20% 100 100 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Acetone 20% 100 100 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Chloroform 20% 100 69 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Dimethylformamide (DMF) 20% 100 74 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Dimethyl Sulfoxide (DMSO) 20% 100 85 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)
Activity - Classical Activity measured by HPLC (nucleoside production measurement) Ethyl Acetate 20% 100 83 % 50 mM MES buffer 6 40Β°C 10 mM Adenine, 10 mM Deoxyadenosine Deoxyadenosine , Hypoxanthine β€” 300 rpm Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*