Esterase (gene estA) from Enterobacter cloacae

Enzyme Description

Species

Enterobacter cloacae ↗

Extremophile

No but"cold-adapted" enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 238 amino acids

MIEIEIRRLGDHEILHATPTGKRDKPLPVVVFYHGFTSSKLVYSYFAVALAQAGFRVVMPDAPDHGVRFTGDEQARLGQFWPILHGSLTEFSGLRDALYDAGLVADNRLAVAGASMGGMTALGIMTHHPEVRSVACLMGSGYFTSLAKTLFPPQDLTEMTARLADWEVTRALPRVADRPLLLWHGDADDVVPPDGTFRLQQALKREGLDGNLTCLWESGVRHRITPAALEATVAFFRQ

Mengmeng Ke et al. (2018) — Engineering and characterization of a novel low temperature active and thermo stable esterase from marine Enterobacter cloacae

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2018.05.193 ↗ · Activity - Classical

18 measurements ▼

Database ID

UniProt: A0ABX9F5R3 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	106.4	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	30%	100.0	11.2	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100.0	62.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	111.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	30%	100.0	74.2	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	30%	100.0	21.7	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	30%	100.0	35.5	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	20%	100.0	20.0	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	102.2	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	97.3	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	100.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	85.4	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100.0	93.8	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	10%	100.0	24.1	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	105.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	99.4	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	114.3	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	107.3	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100.0	106.4	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	30%	100.0	11.2	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100.0	62.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100.0	111.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	30%	100.0	74.2	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	30%	100.0	21.7	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	30%	100.0	35.5	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	20%	100.0	20.0	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100.0	102.2	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	100.0	97.3	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100.0	100.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	100.0	85.4	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	100.0	93.8	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethylene Glycol	10%	100.0	24.1	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100.0	105.6	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100.0	99.4	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100.0	114.3	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	100.0	107.3	%	phosphate-citrate buffer	9	25°C	0.1 mM p-Nitrophenol acetate	Acetic Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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