3-hydroxy-3-methylglutaryl coenzyme A reductase from Sulfolobus solfataricus

Enzyme Description

Species

Sulfolobus solfataricus ↗

Extremophile

Yes "thermoacidophilic" organism

EC Number

1.1.1.34 ↗

Sequence

Length: 409 amino acids

MKIDEVVEKLVKGEISFHEVDNLLEANAAMVARRLALEKIVGVGLPSIGSTVIDYSEIKNKNAENVIGAIQIPLGIVGPIRVNGDYAKGDFYVPMATTEGALIASVNRGIKAVTLSGGVRAKVLKDEMTRAPVFKFDSIEQIPNFLKFIEENLEKIRNIANSTSHHGKLKSITPFVLGNNVWLRFSFETGDAMGMNMVTIAVEKVCEFIEENFPSADCLAVSGNMCSDKKQTNVNSLFGRGKTVVAEALIKKDVIRNILHSNAQLIHDINLRKNWLGTARAGSLSQFNAHFANIVTAIFIATGQDVAQIVESSSGYTWTEVRGEDLYISVTLPSLEVGTVGGGTRLPTQKEALSIMGVYGSGNPPGSNAKKLAEIIASTVLSGELNLLAALSNKELGKAHAKLGRAMKV

Michael Dirkmann et al. (2017) — A Multiperspective Approach to Solvent Regulation of Enzymatic Activity: HMG-CoA Reductase

ChemBioChem · doi:10.1002/cbic.201700596 ↗ · Activity - Classical

79 measurements ▼

Database ID

UniProt: O08424 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli Rosetta‐gamiTM 2(DE3)

Experimental Data (79 measurements)

79 measurements — page 1 of 4

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	30%	100	59	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	66	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	80	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	85	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	93	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2.5%	100	98	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	100	100	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	60%	100	12	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	50%	100	26	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	40%	100	50	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	51	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	20%	100	79	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	15%	100	86	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	10%	100	90	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	5%	100	95	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	2.5%	100	96	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	60%	100	17	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	50%	100	29	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	40%	100	48	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	30%	100	57	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A , Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate

Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	30%	100	59	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	100	66	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	80	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	85	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	5%	100	93	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	2.5%	100	98	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	100	100	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	60%	100	12	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	50%	100	26	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	40%	100	50	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	30%	100	51	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	20%	100	79	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	15%	100	86	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	10%	100	90	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	5%	100	95	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Ethanol	2.5%	100	96	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	60%	100	17	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	50%	100	29	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	40%	100	48	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	30%	100	57	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	40%	100	31	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	60%	100	18	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	50%	100	28	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	40%	100	49	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	30%	100	50	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	20%	100	69	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	10%	100	76	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetone	5%	100	88	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	60%	100	20	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	50%	100	20	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	20%	100	72	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	30%	100	66	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	20%	100	70	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	15%	100	76	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	10%	100	85	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	5%	100	90	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Isopropanol	2.5%	100	96	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	60%	100	20	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	50%	100	22	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	35	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	5%	100	109	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	89	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	2.5%	100	105	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	100	110	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	60%	100	44	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	50%	100	48	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	40%	100	68	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	30%	100	86	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	20%	100	98	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	10%	100	107	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	72	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Tetrahydrofuran (THF)	2.5%	100	106	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	60%	100	35	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	50%	100	51	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	40%	100	52	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	30%	100	77	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	20%	100	101	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	15%	100	106	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	10%	100	117	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	5%	100	111	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	10%	100	72	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	15%	100	78	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	10%	100	80	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	5%	100	92	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Methanol	2.5%	100	97	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	60%	100	20	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	50%	100	22	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	40%	100	30	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	30%	100	44	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	20%	100	55	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Acetonitrile	2.5%	100	105	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	5%	100	84	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	1,4-Dioxane	2.5%	100	89	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100	28	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	25	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	28	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	37	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	47	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate
Activity - Classical	Activity measured by absorbance spectrophotometry (DTNB and released coenzyme A absorbance measurement, 412 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	59	%	100 mM KOAc/100 mM MES buffer, 0.25 mM 5.5-dithio-bis-(2-nitrobenzoic acid)	5.5	45°C	0.25 mM Hydroxymethylglutaroyl coenzyme A	Coenzyme A, Mevalonate	0.5 mM NADPH	—	Classical aqueous control (in %) \| Racemic substrate

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Visualization : Activity — Classical

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Structure

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